{"doi":"10.1016/s1570-0232(02)01042-5","title":"Determination of dextromethorphan and its metabolites in rat serum by liquid–liquid extraction and liquid chromatography with fluorescence detection","abstract":null,"journal":"Journal of Chromatography B","year":2003,"id":640689,"datarank":0.5244761342199721,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"self_citation_contribution":0.5244761342199721,"citation_network_contribution":0.0,"self_endowment_contribution":0.5244761342199721,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":255432,"name":"Bill J. Gurley","orcid":"0000-0001-5377-6388","position":1,"is_corresponding":false},{"id":1665210,"name":"William D. Wessinger","orcid":null,"position":2,"is_corresponding":false},{"id":160337,"name":"Howard P. Hendrickson","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Determination of dextromethorphan and its metabolites in rat serum by liquid–liquid extraction and liquid chromatography with fluorescence detection","abstract":"Dextromethorphan is an effective and safe antitussive, but has liabilities with respect to its abuse potential at doses above the therapeutic dose. At these higher doses, people report phencyclidine-like effects from the drug. A number of animal models have suggested that dextrorphan, an active metabolite of dextromethorphan, is responsible for the abuse liability of the parent compound when dextromethorphan is taken at high doses. Full pharmacokinetic profiles in single animals have not been demonstrated in these studies due to a lack of analytical sensitivity and/or selectivity for dextromethorphan and its metabolites. We have developed a low-cost liquid chromatographic method capable of characterizing the concentration-time profile for dextromethorphan and dextrorphan for 8 h in rats following an 18 mg/kg i.p. dose of dextromethorphan. Limits of quantitation (S/N=10) in 100 microL of serum were 0.25, 0.19, 0.27, and 0.22 nmol/mL for 3-hydroxymorphinan, dextrorphan, 3-methoxymorphinan, and dextromethorphan, respectively. Inter-day precision was better than 11% across the dynamic range of the method.","is_dataset_classified":null,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12705966","pmcid":null,"openalex_id":"https://openalex.org/W1972083277","authors":[],"funders":[{"funder_name":"NIDA NIH HHS","grant_id":"K25 DA014601","title":null},{"funder_name":"NIDA NIH HHS","grant_id":"DA10358","title":null},{"funder_name":"NIDA NIH HHS","grant_id":"R01 DA010358","title":null}],"total_grants":3,"fwci":2.1314,"citation_percentile":0.86317501,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":1},{"year":2014,"count":4},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2017,"count":3},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1570023202010425?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1570023202010425?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/s1570-0232(02)01042-5","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12705966","host_type":"repository"}],"fields_of_study":["Respiratory and Cough-Related Research","Neuropeptides and Animal Physiology","Asthma and respiratory diseases","Animals","Antitussive Agents","Chromatography, Liquid","Dextromethorphan","Rats","Reproducibility of Results","Spectrometry, Fluorescence"],"mesh_terms":["Animals","Antitussive Agents","Chromatography, Liquid","Dextromethorphan","Spectrometry, Fluorescence","Reproducibility of Results","Rats"],"keywords":["Dextrorphan","Dextromethorphan","Chemistry","Metabolite","Chromatography","Active metabolite","Phencyclidine","Pharmacokinetics","Pharmacology","Biochemistry","NMDA receptor","Receptor","Medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T13:16:24.883667Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}