{"doi":"10.1016/s0167-7012(99)00136-0","title":"Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers","abstract":null,"journal":"Journal of Microbiological Methods","year":2000,"id":625142,"datarank":0.8208406010507213,"base_score":5.472270673671475,"endowment":5.472270673671475,"self_citation_contribution":0.8208406010507213,"citation_network_contribution":0.0,"self_endowment_contribution":0.8208406010507213,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":237,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1616393,"name":"Kendall M Gray","orcid":null,"position":1,"is_corresponding":false},{"id":1616392,"name":"Renée S Blosser","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers","abstract":"Fatty acyl homoserine lactones (AHLs) are used as extracellular quorum sensing signals by a variety of gram-negative bacteria. By activating proteins belonging to the LuxR family of transcriptional regulators, these signal metabolites allow population density-dependent gene regulation within a species, as well as interspecies communication among different bacteria. The experimental detection of AHLs is important in the identification of quorum sensing capabilities in bacteria. Chromobacterium violaceum is a gram-negative bacterium that produces the purple pigment violacein in response to the presence of the AHL N-hexanoyl homoserine lactone (C6HSL). The mini-Tn5 mutant strain C. violaceum CV0blu is deficient in the production of this signal molecule but retains the ability to synthesize violacein in response to the presence of C6HSL and a variety of other short-chain AHLs. We have developed a quantitative bioassay that measures the amount of violacein produced by this strain in response to the presence of different concentrations of various AHL molecules. This new assay provides a means of quantifying the amount of a given AHL present in a bacterial culture and can be used to measure differences in AHL production among different strains or different batch cultures of a given species.","is_dataset_classified":null,"base_score":5.472270673671475,"endowment":5.472270673671475,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10739342","pmcid":null,"openalex_id":"https://openalex.org/W2068612391","authors":[],"funders":[],"total_grants":0,"fwci":2.7728,"citation_percentile":0.89004757,"influential_citations":0,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":16},{"year":2014,"count":7},{"year":2015,"count":11},{"year":2016,"count":7},{"year":2017,"count":19},{"year":2018,"count":15},{"year":2019,"count":9},{"year":2020,"count":8},{"year":2021,"count":18},{"year":2022,"count":14},{"year":2023,"count":15},{"year":2024,"count":7},{"year":2025,"count":7},{"year":2026,"count":5}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0167701299001360?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0167701299001360?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/s0167-7012(99)00136-0","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10739342","host_type":"repository"}],"fields_of_study":["Microbial Metabolism and Applications","Bacterial biofilms and quorum sensing","Biochemical and Structural Characterization"],"mesh_terms":["Biological Assay","Chromatography, High Pressure Liquid","Chromobacterium","Indoles","4-Butyrolactone","Signal Transduction","Culture Media, Conditioned"],"keywords":["Chromobacterium violaceum","Quorum sensing","Autoinducer","Homoserine","Bacteria","Biology","Bioassay","Microbiology","Population","Biochemistry","Biofilm","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T05:53:24.345743Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}