{"doi":"10.1016/s0021-9258(19)83641-4","title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties.","abstract":"A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+. The compounds combine an 8-coordinate tetracarboxylate chelating site with stilbene chromophores. Incorporation of the ethylenic linkage of the stilbene into a heterocyclic ring enhances the quantum efficiency and photochemical stability of the fluorophore. Compared to their widely used predecessor, \"quin2\", the new dyes offer up to 30-fold brighter fluorescence, major changes in wavelength not just intensity upon Ca2+ binding, slightly lower affinities for Ca2+, slightly longer wavelengths of excitation, and considerably improved selectivity for Ca2+ over other divalent cations. These properties, particularly the wavelength sensitivity to Ca2+, should make these dyes the preferred fluorescent indicators for many intracellular applications, especially in single cells, adherent cell layers, or bulk tissues.","journal":"Journal of Biological Chemistry","year":1985,"id":12196,"datarank":23.505707596627204,"base_score":9.985436135183296,"endowment":9.985436135183296,"self_citation_contribution":1.4978154202774945,"citation_network_contribution":22.00789217634971,"self_endowment_contribution":1.4978154202774945,"citer_contribution":22.00789217634971,"corpus_percentile":98.7,"corpus_rank":329,"citation_count":21707,"citer_count":194,"citers_with_citation_signal":194,"citers_with_endowment":194,"datacite_reuse_total":0,"is_dataset":false,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1985-03-01","authors":[{"id":96710,"name":"M Poenie","orcid":null,"position":1,"is_corresponding":false},{"id":96711,"name":"R Y Tsien","orcid":null,"position":2,"is_corresponding":false},{"id":96712,"name":"Grzegorz Grynkiewicz","orcid":"0000-0003-2439-2443","position":3,"is_corresponding":false},{"id":96713,"name":"Martin Poenie","orcid":"0000-0003-2524-4039","position":4,"is_corresponding":false},{"id":96714,"name":"Roger Y. Tsien","orcid":null,"position":5,"is_corresponding":false},{"id":96709,"name":"G Grynkiewicz","orcid":null,"position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}