{"doi":"10.1016/j.ymthe.2023.11.017","title":"Circular mRNA-based TCR-T offers a safe and effective therapeutic strategy for treatment of cytomegalovirus infection","abstract":null,"journal":"Molecular Therapy","year":2024,"id":633390,"datarank":0.5244761342199721,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"self_citation_contribution":0.5244761342199721,"citation_network_contribution":0.0,"self_endowment_contribution":0.5244761342199721,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1642124,"name":"Jiali Yang","orcid":null,"position":1,"is_corresponding":false},{"id":1642125,"name":"Chijian Zuo","orcid":null,"position":2,"is_corresponding":false},{"id":1148594,"name":"Jian Xu","orcid":"0000-0001-7033-8574","position":3,"is_corresponding":false},{"id":974771,"name":"Ling Ma","orcid":"0009-0000-3752-8173","position":4,"is_corresponding":false},{"id":1642126,"name":"Qiaomei He","orcid":null,"position":5,"is_corresponding":false},{"id":495779,"name":"Xiao Zhou","orcid":"0000-0001-6510-4095","position":6,"is_corresponding":false},{"id":1642127,"name":"Xiaodan Ding","orcid":null,"position":7,"is_corresponding":false},{"id":1642128,"name":"Lixiang Wei","orcid":null,"position":8,"is_corresponding":false},{"id":1642130,"name":"Suqin Jiang","orcid":null,"position":9,"is_corresponding":false},{"id":1642132,"name":"Luanluan Ma","orcid":null,"position":10,"is_corresponding":false},{"id":1642135,"name":"Benjia Zhang","orcid":null,"position":11,"is_corresponding":false},{"id":760668,"name":"Yuqin Yang","orcid":"0000-0002-2751-5499","position":12,"is_corresponding":false},{"id":1642138,"name":"Baoxia Dong","orcid":null,"position":13,"is_corresponding":false},{"id":1642140,"name":"Liping Wan","orcid":null,"position":14,"is_corresponding":false},{"id":1128814,"name":"Xueying Ding","orcid":"0000-0002-8366-7059","position":15,"is_corresponding":false},{"id":121772,"name":"Ming Zhu","orcid":null,"position":16,"is_corresponding":false},{"id":1642143,"name":"Zhenhua Sun","orcid":null,"position":17,"is_corresponding":false},{"id":1642145,"name":"Pengran Wang","orcid":null,"position":18,"is_corresponding":false},{"id":1592505,"name":"Xianmin Song","orcid":null,"position":19,"is_corresponding":false},{"id":291148,"name":"Yan Zhang","orcid":"0000-0001-8919-1269","position":20,"is_corresponding":false},{"id":1642123,"name":"Lianghua Shen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Circular mRNA-based TCR-T offers a safe and effective therapeutic strategy for treatment of cytomegalovirus infection","abstract":"Circular mRNA (cmRNA) is particular useful due to its high resistance to degradation by exonucleases, resulting in greater stability and protein expression compared to linear mRNA. T cell receptor (TCR)-engineered T cells (TCR-T) represent a promising means of treating viral infections and cancer. This study aimed to evaluate the feasibility and efficacy of cmRNA in antigen-specific-TCR discovery and TCR-T therapy. Using human cytomegalovirus (CMV) pp65 antigen as a model, we found that the expansion of pp65-responsive T cells was induced more effectively by monocyte-derived dendritic cells transfected with pp65-encoding cmRNA compared with linear mRNA. Subsequently, we developed cmRNA-transduced pp65-TCR-T (cm-pp65-TCR-T) that specifically targets the CMV-pp65 epitope. Our results showed that pp65-TCR could be expressed on primary T cells for more than 7 days. Moreover, both in vitro killing and in vivo CDX models demonstrated that cm-pp65-TCR-T cells specifically and persistently kill pp65-and HLA-expressing tumor cells, significantly prolonging the survival of mice. Collectively, our results demonstrated that cmRNA can be used as a more effective technical approach for antigen-specific TCR isolation and identification, and cm-pp65-TCR-T may provide a safe, non-viral, non-integrated therapeutic approach for controlling CMV infection, particularly in patients who have undergone allogeneic hematopoietic stem cell transplantation.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37974400","pmcid":"PMC10787193","openalex_id":null,"authors":[],"funders":[{"funder_name":"Science and Technology Commission of Shanghai Municipality","grant_id":"19JC1414400","title":null},{"funder_name":"Shanghai Shen Kang Hospital Development Center","grant_id":"SHDC2020CR1012B","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2019YFA0111000","title":null},{"funder_name":"Shanghai General Hospital","grant_id":"CCTR-2022ZD07","title":null},{"funder_name":"Ministry of Science and Technology of the People's Republic of China","grant_id":"","title":null},{"funder_name":"School of Medicine, Shanghai Jiao Tong University","grant_id":"","title":null}],"total_grants":6,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":null,"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1525001623006184?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1525001623006184?httpAccept=text/plain","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["T-Lymphocytes","Animals","Humans","Mice","Cytomegalovirus","Cytomegalovirus Infections","Receptors, Antigen, T-Cell","Viral Matrix Proteins","Hematopoietic Stem Cell Transplantation"],"keywords":["Cmv Infection","Allo-hsct","Tcr-t","Circular Mrnas","Pp65 Epitope"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T11:58:50.488211Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}