{"doi":"10.1016/j.ymeth.2016.10.003","title":"Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus","abstract":null,"journal":"Methods","year":2017,"id":630045,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1632050,"name":"Marie-Laure Pinel-Marie","orcid":null,"position":1,"is_corresponding":false},{"id":1632051,"name":"Sophie Chat","orcid":null,"position":2,"is_corresponding":false},{"id":1632053,"name":"Reynald Gillet","orcid":null,"position":3,"is_corresponding":false},{"id":1632054,"name":"Brice Felden","orcid":null,"position":4,"is_corresponding":false},{"id":1632049,"name":"Régine Brielle","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus","abstract":"Polysomes are macromolecular complexes made up of multiple ribosomes simultaneously translating a single mRNA into polypeptide chains. Together, the cellular mRNAs translated in this way are referred to 'translatome.' Translation determines a cell's overall gene expression profile. Studying translatome leads to a better understanding of the translational machinery and of its complex regulatory pathways. Given its fundamental role in cell homeostasis and division, bacterial translation is an important target for antibiotics. However, there are no detailed protocols for polysome purification from Staphylococcus aureus, the human pathogen responsible for the majority of multi-drug resistance issues. We therefore developed methods for the isolation of active polysomes, ribosomes, and ribosomal subunits, examining the purity and quality of each fraction and monitoring polysomal activity during protein synthesis. These steps are mandatory for the use of purified S. aureus polysomes and ribosomes for structural studies or for genome-scale analysis of most translated mRNAs.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27729294","pmcid":null,"openalex_id":"https://openalex.org/W2528466903","authors":[],"funders":[{"funder_name":"School of Pharmacy and Medical Sciences of Rennes University","grant_id":"","title":null},{"funder_name":"(Inserm)","grant_id":"","title":null},{"funder_name":"FRM","grant_id":"","title":null},{"funder_name":"(CNRS)","grant_id":"","title":null}],"total_grants":4,"fwci":0.1603,"citation_percentile":0.63009565,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2021,"count":2},{"year":2023,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S104620231630233X?httpAccept=text/plain","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S104620231630233X?httpAccept=text/xml","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ymeth.2016.10.003","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27729294","host_type":"repository"},{"url":"https://univ-rennes.hal.science/hal-01502132","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","Bacteriophages and microbial interactions","Antimicrobial Resistance in Staphylococcus","Cell Fractionation","Electrophoresis, Agar Gel","Microscopy, Electron","Polyribosomes","Protein Biosynthesis","RNA, Messenger","Ribosome Subunits, Large, Bacterial","Ribosome Subunits, Small, Bacterial","Staphylococcus aureus"],"mesh_terms":["Cell Fractionation","Electrophoresis, Agar Gel","Microscopy, Electron","Polyribosomes","RNA, Messenger","Staphylococcus aureus","Protein Biosynthesis","Ribosome Subunits, Small, Bacterial","Ribosome Subunits, Large, Bacterial"],"keywords":["Polysome","Ribosome","Translation (biology)","Protein biosynthesis","Biology","Messenger RNA","Ribosomal RNA","Staphylococcus aureus","Translational regulation","Cell biology","Ribosomal protein","Gene","Computational biology","Biochemistry","RNA","Bacteria","Genetics","Translation","Translatome","Sucrose Gradient Purification"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T20:21:07.984296Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}