{"doi":"10.1016/j.ymeth.2006.08.011","title":"NMR of membrane proteins in micelles and bilayers: The FXYD family proteins","abstract":null,"journal":"Methods","year":2007,"id":618818,"datarank":0.5606504427425053,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"self_citation_contribution":0.5606504427425053,"citation_network_contribution":0.0,"self_endowment_contribution":0.5606504427425053,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1596659,"name":"Xiao-Min Gong","orcid":null,"position":1,"is_corresponding":false},{"id":1596660,"name":"Khang Thai","orcid":null,"position":2,"is_corresponding":false},{"id":572691,"name":"Jinghua Yu","orcid":"0000-0001-5043-0322","position":3,"is_corresponding":false},{"id":376204,"name":"Francesca M. Marassi","orcid":"0000-0003-2695-5844","position":4,"is_corresponding":false},{"id":1596658,"name":"Carla M. Franzin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"NMR of membrane proteins in micelles and bilayers: The FXYD family proteins","abstract":"Determining the atomic resolution structures of membrane proteins is of particular interest in contemporary structural biology. Helical membrane proteins constitute one-third of the expressed proteins encoded in a genome, many drugs have membrane-bound proteins as their receptors, and mutations in membrane proteins result in human diseases. Although integral membrane proteins provide daunting technical challenges for all methods of protein structure determination, nuclear magnetic resonance (NMR) spectroscopy can be an extremely versatile and powerful method for determining their structures and characterizing their dynamics, in lipid environments that closely mimic the cell membranes. Once milligram amounts of isotopically labeled protein are expressed and purified, micelle samples can be prepared for solution NMR analysis, and lipid bilayer samples can be prepared for solid-state NMR analysis. The two approaches are complementary and can provide detailed structural and dynamic information. This paper describes the steps for membrane protein structure determination using solution and solid-state NMR. The methods for protein expression and purification, sample preparation and NMR experiments are described and illustrated with examples from the FXYD proteins, a family of regulatory subunits of the Na,K-ATPase.","is_dataset_classified":null,"base_score":3.7376696182833684,"endowment":3.7376696182833684,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17367712","pmcid":"PMC2920895","openalex_id":"https://openalex.org/W1974633473","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"P30 CA030199","title":null},{"funder_name":"NIBIB NIH HHS","grant_id":"P41 EB002031","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA082864","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA082864","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM065374","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM065374","title":null}],"total_grants":6,"fwci":3.6489,"citation_percentile":0.9330602,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":2},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":2}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1046202306001848?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1046202306001848?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ymeth.2006.08.011","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17367712","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2920895","host_type":"repository"}],"fields_of_study":["Advanced NMR Techniques and Applications","ATP Synthase and ATPases Research","Protein Structure and Dynamics","Amino Acid Sequence","Cell Line, Transformed","Detergents","Gene Expression","Intracellular Signaling Peptides and Proteins","Lipid Bilayers","Membrane Proteins","Micelles","Molecular Sequence Data","Neoplasm Proteins","Nitrogen Isotopes","Nuclear Magnetic Resonance, Biomolecular","Phosphoproteins","Phosphorus Isotopes","Plasmids","Potassium Channels","Protein Structure, Tertiary","Sodium-Potassium-Exchanging ATPase"],"mesh_terms":["Sodium-Potassium-Exchanging ATPase","Amino Acid Sequence","Cell Line, Transformed","Detergents","Lipid Bilayers","Membrane Proteins","Micelles","Molecular Sequence Data","Neoplasm Proteins","Nitrogen Isotopes","Phosphoproteins","Phosphorus Isotopes","Plasmids","Potassium Channels","Gene Expression","Protein Structure, Tertiary","Nuclear Magnetic Resonance, Biomolecular","Intracellular Signaling Peptides and Proteins"],"keywords":["Membrane protein","Integral membrane protein","Lipid bilayer","Protein–lipid interaction","Nuclear magnetic resonance spectroscopy","Structural biology","Peripheral membrane protein","Micelle","Membrane","Biophysics","Chemistry","Solid-state nuclear magnetic resonance","Structural genomics","Nanodisc","Protein structure","Model lipid bilayer","Biochemistry","Biology","Nuclear magnetic resonance","Lipid bilayer phase behavior","Stereochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T05:21:06.626754Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}