{"doi":"10.1016/j.ymben.2018.07.006","title":"Artificial Protein Scaffold System (AProSS): An efficient method to optimize exogenous metabolic pathways in Saccharomyces cerevisiae","abstract":null,"journal":"Metabolic Engineering","year":2018,"id":629235,"datarank":0.5244761342199721,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"self_citation_contribution":0.5244761342199721,"citation_network_contribution":0.0,"self_endowment_contribution":0.5244761342199721,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":497870,"name":"Xiuqi Chen","orcid":"0000-0003-1157-3501","position":1,"is_corresponding":false},{"id":278949,"name":"Yizhi Cai","orcid":"0000-0003-1663-2865","position":2,"is_corresponding":false},{"id":114628,"name":"Junbiao Dai","orcid":null,"position":3,"is_corresponding":false},{"id":919789,"name":"Tianyi Li","orcid":"0000-0002-6075-6411","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Artificial Protein Scaffold System (AProSS): An efficient method to optimize exogenous metabolic pathways in Saccharomyces cerevisiae","abstract":"Scaffold proteins influence cellular signaling by orchestrating multiple enzymes, receptors or ion channels, and could be tailored to enhance the efficiency of biochemical reactions by positioning related enzymes physically together. However, the number of applicable domains remains small, and the construction of scaffold proteins with optimal domain ratio could be tedious and time-consuming. In this study, we outlined a modular design to quickly assemble scaffold proteins using protein interaction domains, which have been constructed into a standardized vector. We generated multiple protein interaction domains and ligands for making artificial scaffold proteins. At the same time, we developed a robust Golden-Gate-based molecular toolkit for the construction of artificial scaffold proteins, allowing a variance of domain types, number, and positions. The synthesized domain-ligand interaction was verified by yeast two-hybrid and split-GFP assays. Using synthetic scaffolds, we demonstrated an increase in the yield of two target products by 29% and 63% respectively. Moreover, we demonstrated that the synthetic scaffold could be applied to rewire the metabolic flux. Our system could be a useful tool for metabolic engineering and beyond.","is_dataset_classified":null,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30010058","pmcid":null,"openalex_id":"https://openalex.org/W2883694650","authors":[],"funders":[{"funder_name":"National Science Fund for Distinguished Young Scholars","grant_id":"31725002","title":null},{"funder_name":"Bureau of International Cooperation, Chinese Academy of Sciences","grant_id":"172644KYSB20170042","title":null},{"funder_name":"Key Research Program of the Chinese Academy of Science","grant_id":"KFZD-SW-215","title":null}],"total_grants":3,"fwci":1.3197,"citation_percentile":0.80289469,"influential_citations":0,"citation_trend":[{"year":2019,"count":2},{"year":2020,"count":9},{"year":2021,"count":5},{"year":2022,"count":4},{"year":2023,"count":5},{"year":2024,"count":5},{"year":2025,"count":2}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.research.manchester.ac.uk/portal/en/publications/artificial-protein-scaffold-system-apross(7998b957-403d-46ba-8798-d10373e867a9).html","host_type":"repository"},{"url":"https://www.research.manchester.ac.uk/portal/en/publications/artificial-protein-scaffold-system-apross(7998b957-403d-46ba-8798-d10373e867a9).html","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S1096717618301836?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1096717618301836?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ymben.2018.07.006","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30010058","host_type":"repository"},{"url":"https://research.manchester.ac.uk/en/publications/7998b957-403d-46ba-8798-d10373e867a9","host_type":"repository"}],"fields_of_study":["Microbial Metabolic Engineering and Bioproduction","Microbial Natural Products and Biosynthesis","RNA and protein synthesis mechanisms","Metabolic Engineering","Protein Engineering","Recombinant Fusion Proteins","Saccharomyces cerevisiae"],"mesh_terms":["Recombinant Fusion Proteins","Saccharomyces cerevisiae","Protein Engineering","Metabolic Engineering"],"keywords":["Scaffold","Scaffold protein","Saccharomyces cerevisiae","Synthetic biology","Computational biology","Metabolic engineering","Protein engineering","Proteomics","Modular design","Yeast","Domain (mathematical analysis)","Biology","Biochemistry","Enzyme","Computer science","Signal transduction","Gene","Mathematics","Golden Gate Assembly","Artificial Scaffold Protein","Protein-protein Interaction Domain"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.12364268.v1","title":"Additional file 1 of Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.12364268","title":"Additional file 1 of Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T17:40:54.895057Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}