{"doi":"10.1016/j.yexcr.2011.08.019","title":"Evidence for non-CpG methylation in mammals","abstract":null,"journal":"Experimental Cell Research","year":2011,"id":625819,"datarank":0.6284482113039639,"base_score":4.189654742026425,"endowment":4.189654742026425,"self_citation_contribution":0.6284482113039639,"citation_network_contribution":0.0,"self_endowment_contribution":0.6284482113039639,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":65,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":277543,"name":"Juleen R. Zierath","orcid":"0000-0001-6891-7497","position":1,"is_corresponding":false},{"id":632659,"name":"Romain Barrès","orcid":"0000-0002-0158-519X","position":2,"is_corresponding":false},{"id":816197,"name":"Jie Yan","orcid":"0000-0001-6877-5193","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Evidence for non-CpG methylation in mammals","abstract":"In mammals, the existence of cytosine methylation on non-CpG sequences is controversial. Here, we adapted a LuminoMetric-based Assay (LUMA) to determine global non-CpG methylation levels in rodent and human tissues. We observed that <1% cytosines in non-CpG motifs were methylated in 3T3-L1 fibroblasts, whereas 7-13% cytosines in non-CpG motifs were methylated in mouse tissues or embryonic fibroblasts. Analysis of cytosine methylation in human, rat, and mouse tissues by bisulfite sequencing revealed non-CpG methylation levels up to 7.5% of all non-CpG cytosines. These levels dropped to 1.5% when a second round of PCR was performed prior to bisulfite sequencing, providing an explanation for the common underestimation of non-CpG methylation levels. Collectively, our results provide evidence that non-CpG methylation exists at substantial levels in mammals.","is_dataset_classified":null,"base_score":4.189654742026425,"endowment":4.189654742026425,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21925168","pmcid":null,"openalex_id":"https://openalex.org/W1975609775","authors":[],"funders":[{"funder_name":"NNF Center for Basic Metabolic Research","grant_id":"Barres Group","title":null},{"funder_name":"European Research Council","grant_id":"","title":null},{"funder_name":"Knut och Alice Wallenbergs Stiftelse","grant_id":"","title":null},{"funder_name":"Novo Nordisk UK Research Foundation","grant_id":"","title":null}],"total_grants":4,"fwci":2.3113,"citation_percentile":0.88342107,"influential_citations":0,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":7},{"year":2014,"count":6},{"year":2015,"count":8},{"year":2016,"count":1},{"year":2017,"count":9},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2023,"count":1},{"year":2024,"count":16},{"year":2026,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0014482711003429?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0014482711003429?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.yexcr.2011.08.019","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21925168","host_type":"repository"},{"url":"https://researchprofiles.ku.dk/da/publications/eafb1e0d-ca16-4880-87c2-091a377eeca4","host_type":"repository"}],"fields_of_study":["Epigenetics and DNA Methylation","Birth, Development, and Health","Neuroscience of respiration and sleep","3T3-L1 Cells","Animals","Cells, Cultured","CpG Islands","Cytosine","DNA Cleavage","DNA Methylation","Fibroblasts","Humans","Luminescent Measurements","Mice","Muscle, Skeletal","Polymerase Chain Reaction","Rats","Sequence Analysis, DNA","Sulfites"],"mesh_terms":["Animals","Cells, Cultured","Cytosine","Fibroblasts","Humans","Luminescent Measurements","Sulfites","Polymerase Chain Reaction","Sequence Analysis, DNA","Muscle, Skeletal","CpG Islands","DNA Methylation","3T3-L1 Cells","Mice","Rats","DNA Cleavage"],"keywords":["Biology","Methylation","CpG site","DNA methylation","Evolutionary biology","Genetics","Computational biology","Gene","Gene expression"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T11:00:02.721617Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}