{"doi":"10.1016/j.ydbio.2022.08.010","title":"TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line","abstract":"Many tissues contain multipotent stem cells that are critical for maintaining tissue function. In Caenorhabditis elegans, germline stem cells allow gamete production to continue in adulthood. In the gonad, GLP-1/Notch signaling from the distal tip cell niche to neighboring germ cells activates a complex regulatory network to maintain a stem cell population. GLP-1/Notch signaling positively regulates production of LST-1 and SYGL-1 proteins that, in turn, interact with a set of PUF/FBF proteins to positively regulate the stem cell fate. We previously described sog (suppressor of glp-1 loss of function) and teg (tumorous enhancer of glp-1 gain of function) genes that limit the stem cell fate and/or promote the meiotic fate. Here, we show that sog-10 is allelic to nhl-2. NHL-2 is a member of the conserved TRIM-NHL protein family whose members can bind RNA and ubiquitinate protein substrates. We show that NHL-2 acts, at least in part, by inhibiting the expression of PUF-3 and PUF-11 translational repressor proteins that promote the stem cell fate. Two other negative regulators of stem cell fate, CGH-1 (conserved germline helicase) and ALG-5 (Argonaute protein), may work with NHL-2 to modulate the stem cell population. In addition, NHL-2 activity promotes the male germ cell fate in XX animals.","journal":"Developmental Biology","year":2022,"id":282646,"datarank":0.36576973929449247,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.05385350804251705,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.05385350804251705,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":7,"citers_with_citation_signal":5,"citers_with_endowment":5,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9449,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":959088,"name":"Erin M. Jyo","orcid":null,"position":1,"is_corresponding":false},{"id":348754,"name":"Ariz Mohammad","orcid":"0000-0002-2807-412X","position":2,"is_corresponding":false},{"id":958630,"name":"Paul Fox","orcid":"0000-0002-5626-9637","position":3,"is_corresponding":false},{"id":958631,"name":"Vovanti Jones","orcid":"0000-0003-4402-1471","position":4,"is_corresponding":false},{"id":1278,"name":"Elaine R. Mardis","orcid":"0000-0002-5892-1553","position":5,"is_corresponding":false},{"id":246656,"name":"Tim Schedl","orcid":"0000-0003-2148-2996","position":6,"is_corresponding":false},{"id":698444,"name":"Eleanor M. Maine","orcid":"0000-0002-6569-2701","position":7,"is_corresponding":false},{"id":959087,"name":"John L. Brenner","orcid":null,"position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:29:19.691592Z","pmid":"36063869","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}