{"doi":"10.1016/j.xpro.2025.104186","title":"Covalent anchoring of tethered ligands to chemogenetic handles for targeted in vivo neuropharmacology","abstract":"Covalent anchoring of tethered ligands to chemogenetic handles (CATCH) is a chemogenetic approach that enables sustained, localized, and pharmacologically specific receptor antagonism. Here, we present a protocol for designing and applying CATCH in mice. We describe how to integrate CATCH with ex vivo and in vivo electrophysiological recordings, as well as detail procedures for combining CATCH with behavioral assays. CATCH enables the specific control of neurotransmitter receptor function, offering a tool for both neurophysiology and behavioral studies. For complete details on the use and execution of this protocol, please refer to Jehl et al. 1 • CATCH enables precise, long-lasting inhibition of specific brain receptors • Instructions for ex vivo electrophysiology using CATCH in brain slices • Steps for in vivo acute electrophysiology coupled with CATCH • Guide to applying CATCH in vivo for behavioral studies in mice Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Covalent anchoring of tethered ligands to chemogenetic handles (CATCH) is a chemogenetic approach that enables sustained, localized, and pharmacologically specific receptor antagonism. Here, we present a protocol for designing and applying CATCH in mice. We describe how to integrate CATCH with ex vivo and in vivo electrophysiological recordings, as well as detail procedures for combining CATCH with behavioral assays. CATCH enables the specific control of neurotransmitter receptor function, offering a tool for both neurophysiology and behavioral studies.","journal":"STAR Protocols","year":2025,"id":535383,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1419489,"name":"Kian Stenbolt","orcid":null,"position":1,"is_corresponding":false},{"id":1381954,"name":"Alexandre Mourot","orcid":"0000-0002-8839-7481","position":2,"is_corresponding":false},{"id":1381949,"name":"Nicolas Guyon","orcid":"0000-0003-1287-8606","position":3,"is_corresponding":false},{"id":1381948,"name":"Maria Ciscato","orcid":"0009-0006-3373-7860","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":null,"created_at":"2026-07-19T02:51:56.297114Z","pmid":"41223059","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}