{"doi":"10.1016/j.xpro.2025.104112","title":"Protocol for immunofluorescence imaging of epitope-tagged protein in Arabidopsis shoot apical meristem","abstract":"Proteins expressed in or translocated to the shoot apical meristem (SAM) often play pivotal roles in regulating plant development. Here, we present a step-by-step protocol for immunofluorescence imaging to analyze epitope (FLAG)-tagged proteins in the Arabidopsis SAM. This technique may be suitable to visualize mobile proteins because small epitope tags are less disruptive than a relatively large fluorescent protein fusion. For complete details on the use and execution of this protocol, please refer to Takagi et al. 1 • Steps for paraffin fixation of the shoot apical meristem (SAM) of Arabidopsis thaliana • Instructions for cross-sectioning of the SAM in the paraffin block • Guidelines for immunostaining and imaging of FLAG-tagged protein in the SAM Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Proteins expressed in or translocated to the shoot apical meristem (SAM) often play pivotal roles in regulating plant development. Here, we present a step-by-step protocol for immunofluorescence imaging to analyze epitope (FLAG)-tagged proteins in the Arabidopsis SAM. This technique may be suitable to visualize mobile proteins because small epitope tags are less disruptive than a relatively large fluorescent protein fusion.","journal":"STAR Protocols","year":2025,"id":576213,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.957,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1366775,"name":"Naoyuki Uchida","orcid":"0000-0002-4123-6154","position":1,"is_corresponding":false},{"id":292377,"name":"Takato Imaizumi","orcid":"0000-0001-9396-4412","position":2,"is_corresponding":false},{"id":1016350,"name":"Hiroshi Takagi","orcid":"0000-0003-0321-0284","position":0,"is_corresponding":true}],"reference_count":6,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:57:56.636458Z","pmid":"41014567","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}