{"doi":"10.1016/j.xpro.2025.104049","title":"Protocol for plasma membrane enrichment by ultracentrifugation for mass spectrometry of cell lines, xenografts, and patient tumors","abstract":"The success of immunotherapies hinges on identifying targetable cell surface proteins expressed in the cancer of interest. Here, we present a protocol for enriching plasma membrane proteins for mass spectrometry analysis using a density gradient ultracentrifugation approach. We describe steps for cell lysis, membrane isolation, and preparation for downstream analysis. This protocol is applicable to cell lines, cell-/patient-derived xenografts (CDX/PDX), and primary tissues. For complete details on the use and execution of this protocol, please refer to Glisovic-Aplenc et al., 1 Hamilton et al., 2 and Mooney et al. 3","journal":"STAR Protocols","year":2025,"id":546462,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.959,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1060840,"name":"Brian Mooney","orcid":"0009-0003-2834-9781","position":1,"is_corresponding":false},{"id":797244,"name":"Tina Glisovic‐Aplenc","orcid":"0000-0002-8403-1570","position":2,"is_corresponding":false},{"id":613881,"name":"Alexander B. Radaoui","orcid":"0000-0001-5179-7169","position":3,"is_corresponding":false},{"id":274164,"name":"Karina L. Conkrite","orcid":"0000-0002-2064-6947","position":4,"is_corresponding":false},{"id":1438592,"name":"Caitlyn de Jong","orcid":null,"position":5,"is_corresponding":false},{"id":247704,"name":"Simone Sidoli","orcid":"0000-0001-9073-6641","position":6,"is_corresponding":false},{"id":240512,"name":"Poul H. Sorensen","orcid":"0000-0001-7447-2199","position":7,"is_corresponding":false},{"id":74960,"name":"Benjamin A. Garcia","orcid":"0000-0003-3596-4750","position":8,"is_corresponding":false},{"id":85409,"name":"Richard Aplenc","orcid":"0000-0001-7482-5644","position":9,"is_corresponding":false},{"id":240511,"name":"John M. Maris","orcid":"0000-0002-8088-7929","position":10,"is_corresponding":false},{"id":22222,"name":"Gregg B. Morin","orcid":"0000-0001-8949-4374","position":11,"is_corresponding":false},{"id":32676,"name":"Sharon J. Diskin","orcid":"0000-0002-7200-8939","position":12,"is_corresponding":false},{"id":1225686,"name":"Amber K Hamilton","orcid":null,"position":0,"is_corresponding":true}],"reference_count":8,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:53:32.285899Z","pmid":"40884789","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}