{"doi":"10.1016/j.xpro.2025.104046","title":"Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice","abstract":"Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. We present a protocol for tumor imaging in mice using an activatable covalent photoacoustic imaging probe, NOx-JS013. We describe steps for synthesizing NOx-JS013, in vitro and in situ validation through gel-based activity-based protein profiling and cellular imaging, and tumor imaging of aggressive prostate cancer mouse models. This approach provides a strategy for mitigating background noise in the development of photoacoustic imaging probes. For complete details on the use and execution of this protocol, please refer to Song et al. 1","journal":"STAR Protocols","year":2025,"id":573189,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":424932,"name":"Tianqu Zhai","orcid":"0000-0002-1217-5022","position":1,"is_corresponding":false},{"id":411375,"name":"Janggun Jo","orcid":"0000-0001-8131-7197","position":2,"is_corresponding":false},{"id":361954,"name":"Jae Won Chang","orcid":"0000-0003-2397-2497","position":3,"is_corresponding":false},{"id":669104,"name":"Jiho Song","orcid":"0000-0002-0528-8096","position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:57:32.445190Z","pmid":"40849913","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}