{"doi":"10.1016/j.xpro.2025.104013","title":"Protocol for characterizing biomolecular condensates through live-cell imaging and analysis","abstract":"Biomolecular condensates are important targets of investigation due to their physiological functions as well as their roles in neurodegenerative diseases, cancer, and drug delivery. Here, we present a protocol for characterizing biomolecular condensates across three confocal microscopy-based modalities, namely, subcellular localization, fluorescent recovery after photobleaching (FRAP), and time lapse analysis of mobility and fission/fusion events. We detail optimized image acquisition parameters and describe steps for analysis workflows utilizing ImageJ macros and plugins, including 2D and 3D analysis approaches.","journal":"STAR Protocols","year":2025,"id":571871,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9529,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1217311,"name":"Cindy Maurel","orcid":"0000-0003-1487-9987","position":1,"is_corresponding":false},{"id":862901,"name":"Alison Hogan","orcid":"0000-0002-5987-0639","position":2,"is_corresponding":false},{"id":1217313,"name":"Rowan A. W. Radford","orcid":"0000-0002-7906-6795","position":3,"is_corresponding":false},{"id":446966,"name":"Albert Lee","orcid":"0000-0001-5156-0567","position":4,"is_corresponding":false},{"id":1113695,"name":"Roger S. Chung","orcid":"0000-0002-9286-947X","position":5,"is_corresponding":false},{"id":1217315,"name":"Marco Morsch","orcid":"0000-0001-9484-8050","position":6,"is_corresponding":false},{"id":1217312,"name":"G. Richter","orcid":"0000-0002-2352-4224","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:57:19.669553Z","pmid":"40779395","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}