{"doi":"10.1016/j.xpro.2025.103977","title":"Protocol for detecting LDLR on the cell surface of primary mouse hepatocytes using cell-surface biotinylation","abstract":"Here, we present a protocol for selective biotinylation, solubilization, and enrichment of plasma membrane proteins from primary mouse hepatocytes. We describe steps for injecting C57/BL6 mice via the tail vein with adeno-associated virus subtype 8 (AAV8) to specifically knock down the target gene in hepatocytes. We then detail procedures for labeling surface proteins with EZ-LINK Sulfo-NHS-LC-Biotin and enriching low-density lipoprotein receptor (LDLR) with immunoprecipitation. Finally, we outline procedures for assessing surface LDLR with streptavidin-HRP. For complete details on the use and execution of this protocol, please refer to Ma et al. 1","journal":"STAR Protocols","year":2025,"id":570774,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":246025,"name":"Bishuang Cai","orcid":"0000-0002-2047-9923","position":1,"is_corresponding":false},{"id":258780,"name":"Fanglin Ma","orcid":"0000-0002-7251-9544","position":0,"is_corresponding":true}],"reference_count":2,"raw_metadata":null,"created_at":"2026-07-19T02:57:11.713851Z","pmid":"40705595","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}