{"doi":"10.1016/j.xpro.2025.103699","title":"Protocol for mouse carotid artery perfusion for in situ brain tissue fixation and parallel unfixed tissue collection","abstract":"As the study of central control of multiple organ function becomes more prominent, there is an increasing need for the collection of fixed brain and unfixed organs and tissues from the same experimental animal. Here, we present a protocol for performing carotid artery cannulation, organ and tissue collection, in situ brain perfusion and fixation, and brain dissection in mice. We describe steps for cannulating the carotid artery, harvesting the heart and other organs, and perfusing, fixing, and dissecting the brain. • Studies of central control of organ function need fixed brain and other unfixed tissues • Instructions for performing mouse carotid artery cannulation surgery • Steps for cannulating the carotid artery and perfusing, fixing, and dissecting the brain • Guidance on concurrent in situ brain tissue fixation and unfixed tissue collection Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. As the study of central control of multiple organ function becomes more prominent, there is an increasing need for the collection of fixed brain and unfixed organs and tissues from the same experimental animal. Here, we present a protocol for performing carotid artery cannulation, organ and tissue collection, in situ brain perfusion and fixation, and brain dissection in mice. We describe steps for cannulating the carotid artery, harvesting the heart and other organs, and perfusing, fixing, and dissecting the brain.","journal":"STAR Protocols","year":2025,"id":562998,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.942,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":996467,"name":"Zubayer Ibne Ferdous","orcid":"0000-0003-1464-677X","position":1,"is_corresponding":false},{"id":273117,"name":"Pauline Trinh","orcid":null,"position":2,"is_corresponding":false},{"id":1465846,"name":"Valeriy Timofeev","orcid":null,"position":3,"is_corresponding":false},{"id":678429,"name":"Daphne A. Diloretto","orcid":"0000-0002-2386-2119","position":4,"is_corresponding":false},{"id":1416745,"name":"Ning Zong","orcid":null,"position":5,"is_corresponding":false},{"id":1416299,"name":"Chaoyin Chen","orcid":"0000-0001-6114-230X","position":6,"is_corresponding":false},{"id":310948,"name":"Nipavan Chiamvimonvat","orcid":"0000-0001-9499-8817","position":7,"is_corresponding":false},{"id":376798,"name":"Xiao-Dong Zhang","orcid":"0000-0002-1042-8823","position":8,"is_corresponding":false},{"id":310949,"name":"Phung N. Thai","orcid":"0000-0001-7344-150X","position":0,"is_corresponding":true}],"reference_count":13,"raw_metadata":null,"created_at":"2026-07-19T02:56:09.638214Z","pmid":"40088450","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}