{"doi":"10.1016/j.xpro.2025.103692","title":"Protocol for culturing neurospheres from progenitor cells in the dentate gyrus of aged mouse hippocampus","abstract":"The neurosphere assay is the gold standard for assessing the proliferative and differentiation capacities of neural progenitor cells (NPCs). Here, we present a protocol for isolating, propagating, and maintaining hippocampal neurospheres from adult and aged mice and differentiating cultured NPCs into neurons and astrocytes. We describe steps for establishing a heterochronic co-culture of neurosphere-derived cells with primary neurons. Using neurospheres from old animals enables investigation of the effects of aging on the development and differentiation of newborn neurons.","journal":"STAR Protocols","year":2025,"id":562969,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9587,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1335466,"name":"Poommaree Namchaiw","orcid":"0000-0003-0275-6234","position":1,"is_corresponding":false},{"id":402662,"name":"Karl D. Murray","orcid":"0000-0001-8159-6843","position":2,"is_corresponding":false},{"id":513018,"name":"Elva Dı́az","orcid":"0000-0002-3287-7515","position":3,"is_corresponding":false},{"id":402665,"name":"Hwai-Jong Cheng","orcid":"0000-0003-0647-0567","position":4,"is_corresponding":false},{"id":566414,"name":"Olga Vafaeva","orcid":"0000-0002-1909-2596","position":0,"is_corresponding":true}],"reference_count":14,"raw_metadata":null,"created_at":"2026-07-19T02:56:09.638214Z","pmid":"40085650","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}