{"doi":"10.1016/j.xpro.2025.103672","title":"Protocol to purify and culture human pancreatic cancer cells from patient-derived xenografts","abstract":"Pancreatic ductal adenocarcinoma (PDAC) exhibits extensive inter- and intratumoral heterogeneity, along with a significant stromal component. This protocol outlines steps to generate patient-derived xenografts (PDXs), isolate and enrich human PDAC epithelial cells, and verify their identity using droplet digital PCR (ddPCR) and human-specific markers. It provides a robust approach for culturing cancer epithelial cells to develop a human PDAC model system. For complete details on the use and execution of this protocol, please refer to Grygoryev et al. 1 • Steps to expand patient-derived xenograft (PDX) tumors in mice • Details on extracting and dissociating tumors from patient-derived xenografts • Instructions for separating human pancreatic cancer cells from mouse fibroblast cells • Steps to characterize human pancreatic cancer cells derived from PDX Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Pancreatic ductal adenocarcinoma (PDAC) exhibits extensive inter- and intratumoral heterogeneity, along with a significant stromal component. This protocol outlines steps to generate patient-derived xenografts (PDXs), isolate and enrich human PDAC epithelial cells, and verify their identity using droplet digital PCR (ddPCR) and human-specific markers. It provides a robust approach for culturing cancer epithelial cells to develop a human PDAC model system.","journal":"STAR Protocols","year":2025,"id":540546,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9365,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":739178,"name":"Dmytro Grygoryev","orcid":null,"position":1,"is_corresponding":false},{"id":1428599,"name":"Marilynn Chow-Castro","orcid":null,"position":2,"is_corresponding":false},{"id":738499,"name":"Taelor Ekstrom","orcid":"0000-0001-9829-9570","position":3,"is_corresponding":false},{"id":683675,"name":"Elise C. Manalo","orcid":"0000-0003-2752-5280","position":4,"is_corresponding":false},{"id":1428268,"name":"Seung-Won Lee","orcid":"0000-0001-8893-8332","position":5,"is_corresponding":false},{"id":1233993,"name":"Mark Berry","orcid":"0000-0002-8534-2012","position":6,"is_corresponding":false},{"id":626485,"name":"Jason M. Link","orcid":"0000-0002-6892-0431","position":7,"is_corresponding":false},{"id":626486,"name":"Dove Keith","orcid":"0000-0001-6104-3264","position":8,"is_corresponding":false},{"id":1226231,"name":"Brittany L. Allen-Petersen","orcid":"0000-0002-7436-9412","position":9,"is_corresponding":false},{"id":284345,"name":"Brett C. Sheppard","orcid":"0000-0002-2182-7547","position":10,"is_corresponding":false},{"id":305760,"name":"Terry K. Morgan","orcid":"0000-0003-0742-5012","position":11,"is_corresponding":false},{"id":284346,"name":"Rosalie C. Sears","orcid":"0000-0003-1558-2413","position":12,"is_corresponding":false},{"id":506848,"name":"Jung-Sun Kim","orcid":"0000-0003-2454-8065","position":13,"is_corresponding":false},{"id":1428598,"name":"Julien Tessier","orcid":null,"position":0,"is_corresponding":true}],"reference_count":12,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:52:38.861025Z","pmid":"40048424","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}