{"doi":"10.1016/j.xpro.2025.103620","title":"Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique","abstract":null,"journal":"STAR Protocols","year":2025,"id":613156,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1579409,"name":"Adrian Brandtner","orcid":null,"position":1,"is_corresponding":false},{"id":1579412,"name":"Sarah Rieck","orcid":null,"position":2,"is_corresponding":false},{"id":241159,"name":"Bernd K. Fleischmann","orcid":"0000-0002-9202-8363","position":3,"is_corresponding":false},{"id":270708,"name":"Daniela Wenzel","orcid":"0000-0003-1100-6363","position":4,"is_corresponding":false},{"id":1579406,"name":"Alexander Brückner","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique","abstract":"Endothelial cells (ECs) play a major role in vascular homeostasis and pathology. They show a pronounced heterogeneity between different organs as well as within the same organ. Here, we present a protocol for the rapid site-specific isolation of murine aortic ECs. We describe steps for vessel preparation and the isolation of EC layers by freezing on glass coverslips using a modified Häutchen technique. This protocol can be adapted to investigate surface cells from other tissues as well. For complete details on the use and execution of this protocol, please refer to Brückner et al. 1 • Guide for dissection of the mouse aorta • Steps for rapid isolation of aortic endothelial cells • Protocol for RNA isolation, immunostaining, and qPCR for aortic endothelial cells Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Endothelial cells (ECs) play a major role in vascular homeostasis and pathology. They show a pronounced heterogeneity between different organs as well as within the same organ. Here, we present a protocol for the rapid site-specific isolation of murine aortic ECs. We describe steps for vessel preparation and the isolation of EC layers by freezing on glass coverslips using a modified Häutchen technique. This protocol can be adapted to investigate surface cells from other tissues as well.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39921861","pmcid":"PMC11851281","openalex_id":"https://openalex.org/W4407199271","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB TRR259","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"},{"funder_name":"German Research Foundation","grant_id":"","title":null}],"total_grants":3,"fwci":0.4074,"citation_percentile":0.54782757,"influential_citations":0,"citation_trend":[{"year":2025,"count":1}],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://doi.org/10.1016/j.xpro.2025.103620","host_type":"journal"},{"url":"https://doi.org/10.1016/j.xpro.2025.103620","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2666166725000267?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2666166725000267?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39921861","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11851281","host_type":"repository"},{"url":"https://doaj.org/article/6c70a642bb4d496999ee8d879be0b3ca","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11851281/pdf/main.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11851281","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11851281?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1016/j.xpro.2025.103620","host_type":""}],"fields_of_study":["Angiogenesis and VEGF in Cancer","Cell Adhesion Molecules Research","3D Printing in Biomedical Research","Medicine","Biology"],"mesh_terms":["Animals","Aorta","Cell Separation","Endothelial Cells","Mice"],"keywords":["Isolation (microbiology)","Protocol (science)","Computer science","Computational biology","Biology","Cell biology","Chemistry","Medicine","Bioinformatics","Pathology","Microscopy","cell isolation","Gene Expression","Health Sciences","Cell Separation/Fractionation","Q1-390","Mice","Science (General)","Protocol","Animals","Endothelial Cells","Cell Separation","Aorta"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T06:51:51.140365Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}