{"doi":"10.1016/j.xpro.2024.103530","title":"Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans","abstract":"Here, we present a protocol for isolating microvessels from fresh or snap-frozen brain tissue from mice and humans, followed by visualization of RNA utilizing RNAscope hybridization for quantification of mRNA. We describe the steps for sample preparation and isolation, fixation, and hybridization. This protocol was specifically designed to integrate with RNAscope in situ hybridization. Although the protocol was developed in a mouse model, it can be optimized for use in other organisms, including human brain samples.","journal":"STAR Protocols","year":2025,"id":542692,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":653097,"name":"Oandy Naranjo","orcid":"0000-0002-4223-6869","position":1,"is_corresponding":false},{"id":653096,"name":"Silvia Torices","orcid":"0000-0001-8979-887X","position":2,"is_corresponding":false},{"id":1303279,"name":"Sarah Schmidlin","orcid":"0000-0002-4328-685X","position":3,"is_corresponding":false},{"id":1054978,"name":"Destiny Tiburcio","orcid":"0009-0000-5218-9851","position":4,"is_corresponding":false},{"id":678970,"name":"Minseon Park","orcid":"0000-0001-6120-8356","position":5,"is_corresponding":false},{"id":254218,"name":"Michał Toborek","orcid":"0000-0003-4475-2119","position":6,"is_corresponding":false},{"id":254214,"name":"Olivia Osborne","orcid":"0000-0002-8289-8429","position":0,"is_corresponding":true}],"reference_count":2,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:53:00.171379Z","pmid":"39772387","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}