{"doi":"10.1016/j.xpro.2024.103353","title":"Protocol for culturing patient-derived organoids of cervical cancer","abstract":"Herein, we present a protocol for culturing patient-derived organoids (PDOs) of cervical cancer that includes workflows for tumor biopsy/resection tissue and cytobrush-sampled cells. We describe steps for PDO culture initiation, including rinsing, gentle dissociation, Lymphoprep separation, and cell assessment, as well as seeding cells from surgical and cytobrush tissue digestion. We then provide guidance on PDO maintenance and passage and techniques for producing conditioned medium. Overall, this protocol serves as a valuable guide for establishing and maintaining cervical cancer PDOs. For complete details on the use and execution of this protocol, please refer to Colbert et al. 1 • Culturing PDOs from cytobrush and surgical resection tissues in cervical cancer • Workflow schemes and timelines to facilitate smooth implementation • Sampling instructions to improve the success rate with low-tissue-volume samples Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Herein, we present a protocol for culturing patient-derived organoids (PDOs) of cervical cancer that includes workflows for tumor biopsy/resection tissue and cytobrush-sampled cells. We describe steps for PDO culture initiation, including rinsing, gentle dissociation, Lymphoprep separation, and cell assessment, as well as seeding cells from surgical and cytobrush tissue digestion. We then provide guidance on PDO maintenance and passage and techniques for producing conditioned medium. Overall, this protocol serves as a valuable guide for establishing and maintaining cervical cancer PDOs.","journal":"STAR Protocols","year":2024,"id":461437,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9455,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1120062,"name":"Timothy Harris","orcid":"0000-0002-3939-6263","position":1,"is_corresponding":false},{"id":978359,"name":"Dalissa Negrón‐Figueroa","orcid":"0000-0002-4200-0733","position":2,"is_corresponding":false},{"id":211798,"name":"David Lo","orcid":null,"position":3,"is_corresponding":false},{"id":399419,"name":"Allison Judge","orcid":"0000-0003-4289-1748","position":4,"is_corresponding":false},{"id":1290381,"name":"D'Shaunique Walters","orcid":null,"position":5,"is_corresponding":false},{"id":1289907,"name":"Bo Jiang","orcid":"0000-0002-7291-3971","position":6,"is_corresponding":false},{"id":292266,"name":"Lauren E. Colbert","orcid":"0000-0001-5806-4339","position":7,"is_corresponding":false},{"id":329457,"name":"Rui Wang","orcid":"0000-0002-3191-9031","position":0,"is_corresponding":true}],"reference_count":7,"raw_metadata":null,"created_at":"2026-07-19T02:04:12.271488Z","pmid":"39356641","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}