{"doi":"10.1016/j.xpro.2024.103111","title":"Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress","abstract":"Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic \"livers in a dish.\" We describe strategies to induce metabolic and alcohol-associated hepatic steatosis, inflammation, and fibrosis. We outline potential applications, including using human liver spheroids for experimental and translational research and drug screening to identify potential anti-fibrotic therapies.","journal":"STAR Protocols","year":2024,"id":430730,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9457,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1028682,"name":"Wonseok Lee","orcid":null,"position":1,"is_corresponding":false},{"id":1234062,"name":"Xiao Liu","orcid":"0000-0001-9131-8548","position":2,"is_corresponding":false},{"id":1234550,"name":"Haeum Jang","orcid":null,"position":3,"is_corresponding":false},{"id":1027810,"name":"Sadatsugu Sakane","orcid":"0000-0002-6306-4621","position":4,"is_corresponding":false},{"id":1205060,"name":"Raquel Carvalho‐Gontijo Weber","orcid":null,"position":5,"is_corresponding":false},{"id":1020647,"name":"Karin Diggle","orcid":null,"position":6,"is_corresponding":false},{"id":289064,"name":"Samuel A. Kerk","orcid":"0000-0001-9786-2245","position":7,"is_corresponding":false},{"id":229039,"name":"Christian M. Metallo","orcid":"0000-0003-2404-3040","position":8,"is_corresponding":false},{"id":226038,"name":"Tatiana Kisseleva","orcid":"0000-0001-9107-2306","position":9,"is_corresponding":false},{"id":232503,"name":"David A. Brenner","orcid":"0000-0003-2573-525X","position":10,"is_corresponding":false},{"id":1027809,"name":"Hyun Young Kim","orcid":"0009-0006-2497-6229","position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-19T01:59:20.861374Z","pmid":"38833372","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}