{"doi":"10.1016/j.xpro.2024.102878","title":"Protocol for neurogenin-2-mediated induction of human stem cell-derived neural progenitor cells","abstract":"Human pluripotent stem cell-derived neural progenitor cells (NPCs) are an essential tool for the study of brain development and developmental disorders such as autism. Here, we present a protocol to generate NPCs rapidly and reproducibly from human stem cells using dual-SMAD inhibition coupled with a brief pulse of mouse neurogenin-2 (Ngn2) overexpression. We detail the 48-h induction scheme deployed to produce these cells—termed stem cell-derived Ngn2-accelerated progenitor cells—followed by steps for expansion, purification, banking, and quality assessment. For complete details on the use and execution of this protocol, please refer to Wells et al.1","journal":"STAR Protocols","year":2024,"id":444155,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9592,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":556355,"name":"Laila Sathe","orcid":"0000-0003-1016-3295","position":1,"is_corresponding":false},{"id":1259694,"name":"Alexander Dai","orcid":null,"position":2,"is_corresponding":false},{"id":1259695,"name":"Tim Derebenskiy","orcid":null,"position":3,"is_corresponding":false},{"id":812649,"name":"Ana Rodriguez Vega","orcid":"0000-0001-7545-9500","position":4,"is_corresponding":false},{"id":33055,"name":"Kevin Eggan","orcid":"0000-0003-4436-8467","position":5,"is_corresponding":false},{"id":232265,"name":"Michael F. Wells","orcid":"0000-0002-1367-2565","position":6,"is_corresponding":false},{"id":859332,"name":"Ellen J. Guss","orcid":"0000-0002-2579-8712","position":0,"is_corresponding":true}],"reference_count":5,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:01:33.526738Z","pmid":"38335091","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}