{"doi":"10.1016/j.xpro.2023.102805","title":"Single-cell RNA sequencing comparison of the human metastatic prostate spine tumor microenvironment","abstract":"Spinal column tumors can be difficult to process for single-cell omic studies, given the heterogeneity in tissue. Here, we present a protocol for operating room-to-benchtop single-cell processing of clinical specimens from a prostate cancer patient. We describe steps for sample homogenization, red blood cell lysis, cryopreservation, and single-cell sequencing analysis. This protocol can be used to identify prognostic markers and therapeutic targets for patients with osseous spine metastases and better inform eligibility for clinical trials.","journal":"STAR Protocols","year":2024,"id":467325,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9255,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":404064,"name":"Corinne Haines","orcid":"0000-0003-0061-6983","position":1,"is_corresponding":false},{"id":325725,"name":"Meghan Price","orcid":"0000-0002-2683-9758","position":2,"is_corresponding":false},{"id":757069,"name":"Tara Dalton","orcid":"0000-0003-4796-6948","position":3,"is_corresponding":false},{"id":762908,"name":"César Baëta","orcid":"0000-0001-6054-5369","position":4,"is_corresponding":false},{"id":1300093,"name":"Harrison Hockenberry","orcid":null,"position":5,"is_corresponding":false},{"id":357014,"name":"C. Rory Goodwin","orcid":"0000-0002-6540-2751","position":6,"is_corresponding":false},{"id":748675,"name":"Annee Nguyen","orcid":"0000-0002-5550-9053","position":0,"is_corresponding":true}],"reference_count":10,"raw_metadata":null,"created_at":"2026-07-19T02:05:10.907983Z","pmid":"38341849","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}