{"doi":"10.1016/j.xpro.2023.102762","title":"Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators","abstract":"Ferroptosis, an iron-dependent programmed cell death triggered by excessive lipid peroxidation, has shown promising therapeutic potentials in human diseases. Here, we describe a protocol of a CRISPR-Cas9 loss-of-function screen to identify regulators in response to different inducers of ferroptosis. We emphasize the steps of library amplification, drug treatment, high-throughput sequencing preparation, and bioinformatics analysis using model-based analysis of genome-wide CRISPR-Cas9 knockout (MAGeCK). We also present a method to discover the regulators of ferroptosis and verify the potential targets efficiently. For complete details on use and execution of this protocol, please refer to Yang et al. (2023).1","journal":"STAR Protocols","year":2023,"id":352067,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":884900,"name":"Shoufu Duan","orcid":"0000-0003-0416-374X","position":1,"is_corresponding":false},{"id":272413,"name":"Zhiming Li","orcid":"0000-0002-5031-8362","position":2,"is_corresponding":false},{"id":1080868,"name":"Zhe Wang","orcid":"0000-0001-5407-4230","position":3,"is_corresponding":false},{"id":282933,"name":"Ning Kon","orcid":"0000-0002-5699-3780","position":4,"is_corresponding":false},{"id":270525,"name":"Zhiguo Zhang","orcid":"0000-0002-9451-2685","position":5,"is_corresponding":false},{"id":486978,"name":"Wei Gu","orcid":"0000-0002-1641-5740","position":6,"is_corresponding":false},{"id":618519,"name":"Xin Yang","orcid":"0000-0003-0222-5839","position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:12:50.107998Z","pmid":"38048220","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}