{"doi":"10.1016/j.xpro.2023.102702","title":"Protocol for the saturation and multiplexing of genetic variants using CRISPR-Cas9","abstract":"Here, we present a multiplexed assay for variant effect protocol to assess the functional impact of all possible genetic variations within a particular genomic region. We describe steps for saturation genome editing by designing and cloning of single-guide RNA (sgRNA). We then detail steps for nucleofection of sgRNA, testing drug response on variants, and amplification of genomic DNA for next-generation sequencing. For complete details on the use and execution of this protocol, please refer to Sahu et al.1","journal":"STAR Protocols","year":2023,"id":373769,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":390850,"name":"Teresa Sullivan","orcid":"0000-0001-8771-9066","position":1,"is_corresponding":false},{"id":391706,"name":"Eileen Southon","orcid":null,"position":2,"is_corresponding":false},{"id":1051581,"name":"Dylan Caylor","orcid":null,"position":3,"is_corresponding":false},{"id":1135452,"name":"Josephine Geh","orcid":null,"position":4,"is_corresponding":false},{"id":271851,"name":"Shyam K. Sharan","orcid":"0000-0002-9333-870X","position":5,"is_corresponding":false},{"id":352571,"name":"Sounak Sahu","orcid":"0000-0001-7290-6651","position":0,"is_corresponding":true}],"reference_count":9,"raw_metadata":null,"created_at":"2026-07-19T01:16:07.080027Z","pmid":"37948185","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}