{"doi":"10.1016/j.xpro.2023.102385","title":"Glyoxal-based fixation of Drosophila embryos for immunofluorescence staining and RNA in situ hybridization","abstract":"The dialdehyde glyoxal is an alternative chemical fixative that cross-links tissues faster than formaldehyde, retains higher antigenicity, and is less hazardous than either formaldehyde or glutaraldehyde. Here we present a glyoxal-based fixation protocol for use with Drosophila embryos. We describe steps to prepare acid-free glyoxal, fix embryos, and then stain with antibodies for immunofluorescence (IF). We also describe methods for RNA fluorescence in situ hybridization (FISH) and FISH plus IF (FISH-IF) using glyoxal-fixed embryos. This protocol was adapted for Drosophila embryos from the methods of Bussolati et al.1 and Richter et al.2","journal":"STAR Protocols","year":2023,"id":375082,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1137759,"name":"Malika Basu","orcid":null,"position":1,"is_corresponding":false},{"id":1094765,"name":"Valeria Buzinova","orcid":null,"position":2,"is_corresponding":false},{"id":1137760,"name":"Anthony Delgado","orcid":null,"position":3,"is_corresponding":false},{"id":1137316,"name":"Tejas Mahadevan","orcid":"0000-0001-8226-1164","position":4,"is_corresponding":false},{"id":1137761,"name":"Sanya Mishra","orcid":null,"position":5,"is_corresponding":false},{"id":1137762,"name":"Sarah Zaida","orcid":null,"position":6,"is_corresponding":false},{"id":669171,"name":"Xi Wang","orcid":"0009-0004-9115-8539","position":7,"is_corresponding":false},{"id":885890,"name":"Anna Marie Sokac","orcid":"0000-0001-7803-3062","position":8,"is_corresponding":false},{"id":1137315,"name":"Shrunali Amin","orcid":"0009-0009-5032-2212","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-19T01:16:19.336238Z","pmid":"37405926","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}