{"doi":"10.1016/j.xpro.2023.102271","title":"Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody","abstract":"The apolipoprotein E protein (apoE) confers differential risk for Alzheimer's disease depending on which isoforms are expressed. Here, we present a 2-day immunoprecipitation protocol using the HJ15.4 monoclonal apoE antibody for the pull-down of native apoE particles. We describe major steps for apoE production via immortalized astrocyte culture and HJ15.4 antibody bead coupling for apoE particle pull-down, elution, and characterization. This protocol could be used to isolate native apoE particles from multiple model systems or human biospecimens.","journal":"STAR Protocols","year":2023,"id":408323,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9579,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":806633,"name":"Ana‐Caroline Raulin","orcid":"0000-0001-5044-9197","position":1,"is_corresponding":false},{"id":233771,"name":"Zonghua Li","orcid":"0000-0001-5242-9608","position":2,"is_corresponding":false},{"id":230884,"name":"Yuka A. Martens","orcid":"0000-0002-5194-4269","position":3,"is_corresponding":false},{"id":1014596,"name":"Yasuteru Inoue","orcid":"0000-0003-4180-5908","position":4,"is_corresponding":false},{"id":233379,"name":"Michael R. Strickland","orcid":"0000-0002-3899-5651","position":5,"is_corresponding":false},{"id":232153,"name":"Xianlin Han","orcid":"0000-0002-8615-2413","position":6,"is_corresponding":false},{"id":27592,"name":"David M. Holtzman","orcid":"0000-0002-3400-0856","position":7,"is_corresponding":false},{"id":230893,"name":"Guojun Bu","orcid":"0000-0003-3491-1016","position":8,"is_corresponding":false},{"id":233772,"name":"Na Zhao","orcid":"0000-0001-8648-9422","position":9,"is_corresponding":false},{"id":617918,"name":"Justin O’Leary","orcid":"0000-0002-7538-8455","position":0,"is_corresponding":true}],"reference_count":11,"raw_metadata":null,"created_at":"2026-07-19T01:21:18.414003Z","pmid":"37289593","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}