{"doi":"10.1016/j.xpro.2023.102262","title":"Optimized protocol for in vivo affinity purification proteomics and biochemistry using C. elegans","abstract":"We present an optimized protocol for in vivo affinity purification proteomics and biochemistry using the model organism C. elegans. We describe steps for target tagging, large-scale culture, affinity purification using a cryomill, mass spectrometry and validation of candidate binding proteins. Our approach has proven successful for identifying protein-protein interactions and signaling networks with verified functional relevance. Our protocol is also suitable for biochemical evaluation of protein-protein interactions in vivo. For complete details on the use and execution of this protocol, please refer to Crawley et al.,1 Giles et al.,2 and Desbois et al.3","journal":"STAR Protocols","year":2023,"id":372890,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9521,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":350190,"name":"Joseph S. Pak","orcid":"0000-0003-2004-9015","position":1,"is_corresponding":false},{"id":757026,"name":"Karla J. Opperman","orcid":"0000-0001-9545-8361","position":2,"is_corresponding":false},{"id":348129,"name":"Andrew C. Giles","orcid":"0000-0002-0875-0696","position":3,"is_corresponding":false},{"id":348130,"name":"Brock Grill","orcid":"0000-0002-0379-3267","position":4,"is_corresponding":false},{"id":882485,"name":"Muriel Desbois","orcid":"0000-0002-8070-2525","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T01:15:58.101083Z","pmid":"37294631","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}