{"doi":"10.1016/j.xpro.2023.102220","title":"Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models","abstract":"The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We describe steps for decalcifying bone, using solvents to remove color and lipids, and imaging tissues in 3D using confocal and light sheet microscopy. We then detail a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software. For complete details on the use and execution of this protocol, please refer to (Moatti et al., 2022).1","journal":"STAR Protocols","year":2023,"id":369512,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9393,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":842411,"name":"Yuheng Cai","orcid":"0009-0000-1619-5437","position":1,"is_corresponding":false},{"id":1128456,"name":"Chen Li","orcid":"0000-0003-3556-2719","position":2,"is_corresponding":false},{"id":80216,"name":"Kristen D. Popowski","orcid":"0000-0002-6341-2251","position":3,"is_corresponding":false},{"id":1128457,"name":"Ke Cheng","orcid":"0000-0002-6876-5398","position":4,"is_corresponding":false},{"id":341733,"name":"Frances S. Ligler","orcid":"0000-0003-4909-2084","position":5,"is_corresponding":false},{"id":245908,"name":"Alon Greenbaum","orcid":"0000-0002-2897-876X","position":6,"is_corresponding":false},{"id":677296,"name":"Adele Moatti","orcid":"0000-0002-6946-5370","position":0,"is_corresponding":true}],"reference_count":13,"raw_metadata":null,"created_at":"2026-07-19T01:15:32.670954Z","pmid":"37060559","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}