{"doi":"10.1016/j.xpro.2023.102148","title":"Protocol to assess substrate dephosphorylation by serine/threonine phosphoprotein phosphatases in vitro","abstract":"Serine/threonine protein phosphatase 2 (PP2A) forms heterotrimeric holoenzymes, where a scaffold subunit bridges the PP2A catalytic subunit to a B regulatory subunit, e.g., B55α. The PP2A/B55α holoenzyme plays key roles in signaling and cell-cycle control targeting multiple substrates. Here, we describe semiquantitative approaches to determine PP2A/B55α substrate specificity. Parts I and II detail approaches to assess PP2A/B55α-mediated dephosphorylation of immobilized substrate peptide variants. Parts III and IV detail methods to assess PP2A/B55α-substrate-binding specificity. These approaches are adaptable to other serine/threonine phosphatases. For complete details on the use and execution of this protocol, please refer to Fowle et al..1","journal":"STAR Protocols","year":2023,"id":388127,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9595,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":812832,"name":"Felicity Feiser","orcid":null,"position":1,"is_corresponding":false},{"id":1157376,"name":"Seren M. Palacio","orcid":"0009-0002-6677-604X","position":2,"is_corresponding":false},{"id":1157377,"name":"Kishan Patel","orcid":"0000-0001-9866-0231","position":3,"is_corresponding":false},{"id":1157378,"name":"Joy Gonzalez","orcid":"0009-0004-2433-3229","position":4,"is_corresponding":false},{"id":365230,"name":"Holly Fowle","orcid":null,"position":5,"is_corresponding":false},{"id":363460,"name":"Xavier Graña","orcid":"0000-0001-7134-0473","position":6,"is_corresponding":false},{"id":496336,"name":"Jason S. Wasserman","orcid":"0000-0002-0697-5971","position":0,"is_corresponding":true}],"reference_count":16,"raw_metadata":null,"created_at":"2026-07-19T01:18:13.977537Z","pmid":"37074907","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}