{"doi":"10.1016/j.xpro.2023.102054","title":"Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling","abstract":"Automated single-cell dispensing is incompatible with white adipose tissue (WAT) due to lipid-laden adipocytes. Single-nuclei RNA-Seq permits transcriptional profiling of all cells from WAT. Human WAT faces unique technical challenges in isolating nuclei compared to rodent tissue due to greater extra-cellular matrix content and larger lipid droplets. In this protocol, we detail how to isolate nuclei from frozen subcutaneous human WAT for single-nuclei RNA-Seq. For complete information on the generation and use of this protocol, please refer to Whytock et al. (2022).1","journal":"STAR Protocols","year":2023,"id":350823,"datarank":0.37535968419922283,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.029971920250115896,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.029971920250115896,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":3,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9358,"is_data_producer":true,"deposit_databanks":{"ClinicalTrials.gov":["NCT04034706"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":499388,"name":"Adeline Divoux","orcid":"0000-0003-1310-2092","position":1,"is_corresponding":false},{"id":2326,"name":"Yifei Sun","orcid":"0000-0002-5530-8833","position":2,"is_corresponding":false},{"id":499390,"name":"Meghan Hopf","orcid":"0000-0001-9767-8325","position":3,"is_corresponding":false},{"id":1061882,"name":"Reichelle X. Yeo","orcid":"0000-0002-2403-6150","position":4,"is_corresponding":false},{"id":997643,"name":"Maria F. Pino","orcid":null,"position":5,"is_corresponding":false},{"id":711422,"name":"Gongxin Yu","orcid":"0000-0001-9658-1038","position":6,"is_corresponding":false},{"id":300728,"name":"Steven R. Smith","orcid":"0000-0002-5098-8147","position":7,"is_corresponding":false},{"id":2335,"name":"Martin J. Walsh","orcid":"0000-0001-8339-8285","position":8,"is_corresponding":false},{"id":235346,"name":"Lauren M. Sparks","orcid":"0000-0002-8724-6016","position":9,"is_corresponding":false},{"id":711419,"name":"Katie L. Whytock","orcid":"0000-0003-3486-9452","position":0,"is_corresponding":true}],"reference_count":16,"raw_metadata":null,"created_at":"2026-07-19T01:12:37.409066Z","pmid":"36853719","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}