{"doi":"10.1016/j.xpro.2022.101871","title":"Quantification of autophagy flux in isolated mouse skeletal muscle fibers with overexpression of fluorescent protein mCherry-EGFP-LC3","abstract":"Evaluation of autophagy flux could be challenging for muscle fibers due to the baseline expression of mCherry-EGFP-LC3 along the Z-line. We established a protocol to overcome this difficulty. We overexpress mChery-EGFP-LC3 in the FDB muscle of an adult mouse via electroporation. Then, we enzymatically digest FDB muscle to yield individual fibers for live cell imaging. Finally, we develop an ImageJ-based program to eliminate the baseline striation pattern and semi-automatically quantify autophagosomes (APs) and autolysosomes (ALs) for autophagy flux analysis.","journal":"STAR Protocols","year":2023,"id":376510,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9517,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1139746,"name":"Ju Hwan Cho","orcid":"0000-0003-2755-0259","position":1,"is_corresponding":false},{"id":496480,"name":"Jianxun Yi","orcid":null,"position":2,"is_corresponding":false},{"id":1140124,"name":"Kyoung‐Han Choi","orcid":null,"position":3,"is_corresponding":false},{"id":665161,"name":"Ki Ho Park","orcid":"0000-0002-6193-9826","position":4,"is_corresponding":false},{"id":554568,"name":"Hua Zhu","orcid":"0000-0001-7136-7326","position":5,"is_corresponding":false},{"id":480434,"name":"Chuanxi Cai","orcid":"0000-0003-0532-7435","position":6,"is_corresponding":false},{"id":667286,"name":"Erin Haggard","orcid":null,"position":7,"is_corresponding":false},{"id":495367,"name":"Jingsong Zhou","orcid":"0000-0002-5684-7177","position":8,"is_corresponding":false},{"id":672278,"name":"Jae‐Kyun Ko","orcid":"0000-0001-9174-5806","position":9,"is_corresponding":false},{"id":341623,"name":"Jianjie Ma","orcid":"0000-0003-3865-6112","position":10,"is_corresponding":false},{"id":1139745,"name":"Xinyu Zhou","orcid":"0000-0002-9647-1100","position":0,"is_corresponding":true}],"reference_count":19,"raw_metadata":null,"created_at":"2026-07-19T01:16:32.368674Z","pmid":"36856767","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}