{"doi":"10.1016/j.xpro.2022.101776","title":"Protocol to dissociate, process, and analyze the human lung tissue using single-cell RNA-seq","abstract":"We report a protocol for obtaining high-quality single-cell transcriptomics data from human lung biospecimens acquired from core needle biopsies, fine-needle aspirates, surgical resection, and pleural effusions. The protocol relies upon the brief mechanical and enzymatic disruption of tissue, enrichment of live cells by fluorescence-activated cell sorting (FACS), and droplet-based single-cell RNA sequencing (scRNA-seq). The protocol also details a procedure for analyzing the scRNA-seq data. For complete details on the use and execution of this protocol, please refer to Chan et al. (2021).","journal":"STAR Protocols","year":2022,"id":263619,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.7881,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":225134,"name":"Joseph M. Chan","orcid":"0000-0001-5249-7395","position":1,"is_corresponding":false},{"id":50787,"name":"Ignas Masilionis","orcid":"0000-0001-7937-9014","position":2,"is_corresponding":false},{"id":238281,"name":"Vianne R. Gao","orcid":"0000-0001-8990-0897","position":3,"is_corresponding":false},{"id":226864,"name":"Yubin Xie","orcid":"0000-0003-2542-2544","position":4,"is_corresponding":false},{"id":229185,"name":"Viola Allaj","orcid":null,"position":5,"is_corresponding":false},{"id":297331,"name":"Andrew Chow","orcid":"0000-0003-1151-2450","position":6,"is_corresponding":false},{"id":65360,"name":"John T. Poirier","orcid":"0000-0001-9795-5644","position":7,"is_corresponding":false},{"id":104368,"name":"Dana Pe’er","orcid":"0000-0002-9259-8817","position":8,"is_corresponding":false},{"id":1555,"name":"Charles M. Rudin","orcid":"0000-0001-5204-3465","position":9,"is_corresponding":false},{"id":50789,"name":"Linas Mazutis","orcid":"0000-0002-5552-6427","position":10,"is_corresponding":false},{"id":238377,"name":"Àlvaro Quintanal-Villalonga","orcid":"0000-0002-7234-3446","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:26:33.413850Z","pmid":"36313536","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}