{"doi":"10.1016/j.xpro.2022.101668","title":"Optimized protocol to isolate primary mouse peritoneal macrophage metabolites","abstract":"Peritoneal macrophages (PMs) have been shown to have higher stability compared to other macrophage subtypes. However, obtaining enough PMs from a single mouse is often a limitation for metabolomics analysis. Here, we describe a protocol to isolate metabolites from a small number of mouse primary PMs for 13C-stable glucose tracing and metabolomics. Our protocol uses X for metabolite extraction instead of methanol. Our protocol can consistently extract metabolites from low cell number samples with fewer steps than methanol-based approaches. For complete details on the use and execution of this protocol, please refer to De Jesus et al., (2022).","journal":"STAR Protocols","year":2022,"id":250115,"datarank":0.49983067652628066,"base_score":3.332204510175204,"endowment":3.332204510175204,"self_citation_contribution":0.49983067652628066,"citation_network_contribution":0.0,"self_endowment_contribution":0.49983067652628066,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":650280,"name":"Carolina Pusec","orcid":"0000-0002-8238-8234","position":1,"is_corresponding":false},{"id":274702,"name":"Tivoli Nguyen","orcid":"0000-0002-3505-5154","position":2,"is_corresponding":false},{"id":892265,"name":"Farnaz Keyhani-Nejad","orcid":null,"position":3,"is_corresponding":false},{"id":230871,"name":"Peng Gao","orcid":"0000-0002-9991-6064","position":4,"is_corresponding":false},{"id":230870,"name":"Samuel E. Weinberg","orcid":"0000-0002-9532-7051","position":5,"is_corresponding":false},{"id":117198,"name":"Hossein Ardehali","orcid":"0000-0002-7662-0551","position":6,"is_corresponding":false},{"id":650281,"name":"Adam De Jesus","orcid":"0000-0002-7491-4732","position":0,"is_corresponding":true}],"reference_count":9,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:24:28.242323Z","pmid":"36103306","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}