{"doi":"10.1016/j.xpro.2022.101665","title":"Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence","abstract":"Previous studies have demonstrated that a high-protein diet leads to increased atherosclerosis in mice, and that this adverse effect is caused by activation of macrophage mTORC1 signaling. Here, we provide a detailed protocol for the evaluation of diet-induced mTORC1 signaling in plaque macrophages in atherosclerosis-prone apolipoprotein E (ApoE) knockout (KO) mice. This protocol includes flow cytometry and immunofluorescence analysis of atherosclerotic macrophages that can be used to study the atherogenic potential of a variety of mTORC1 modulators. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).","journal":"STAR Protocols","year":2022,"id":288283,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9445,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":868178,"name":"Jeremiah Stitham","orcid":null,"position":1,"is_corresponding":false},{"id":802627,"name":"Astrid Rodríguez-Vélez","orcid":"0000-0003-3376-1300","position":2,"is_corresponding":false},{"id":587999,"name":"Se‐Jin Jeong","orcid":"0000-0002-6375-5334","position":3,"is_corresponding":false},{"id":802625,"name":"Arick Park","orcid":"0000-0001-5668-0701","position":4,"is_corresponding":false},{"id":802626,"name":"Yu‐Sheng Yeh","orcid":"0000-0002-0099-2690","position":5,"is_corresponding":false},{"id":736267,"name":"Divya Kapoor","orcid":"0000-0001-7142-3025","position":6,"is_corresponding":false},{"id":304278,"name":"Bettina Mittendorfer","orcid":"0000-0003-0049-2677","position":7,"is_corresponding":false},{"id":241767,"name":"Babak Razani","orcid":"0000-0002-7172-9240","position":8,"is_corresponding":false},{"id":802628,"name":"Xiangyu Zhang","orcid":"0000-0003-0170-0837","position":0,"is_corresponding":true}],"reference_count":3,"raw_metadata":null,"created_at":"2026-07-19T00:30:11.069895Z","pmid":"36094885","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}