{"doi":"10.1016/j.xpro.2022.101160","title":"Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse brain","abstract":"Here, we present a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, applied to the central amygdala oxytocin receptor-expressing astrocytes. This includes RNAse-free perfusion, combination of RNAscope and immunohistochemistry, and confocal imaging. This protocol provides detailed information about tissue handling and a comprehensive description of the RNAScope technique to label rat and mouse oxytocin receptor mRNA. We also describe three-dimensional reconstruction that allows the assessment of more than 70 different cellular parameters, powerful for studying astrocyte morphology and astrocyte-astrocyte interactions. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021) and Althammer et al. (2020).","journal":"STAR Protocols","year":2022,"id":259543,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8776,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":341131,"name":"Eric G. Krause","orcid":null,"position":1,"is_corresponding":false},{"id":340406,"name":"Annette D. de Kloet","orcid":"0000-0002-7213-6957","position":2,"is_corresponding":false},{"id":490149,"name":"Justin A. Smith","orcid":"0000-0003-3835-7622","position":3,"is_corresponding":false},{"id":5873,"name":"Valery Grinevich","orcid":"0000-0002-6337-8866","position":4,"is_corresponding":false},{"id":238033,"name":"Alexandre Charlet","orcid":"0000-0003-4837-3706","position":5,"is_corresponding":false},{"id":289217,"name":"Javier E. Stern","orcid":"0000-0002-9310-0486","position":6,"is_corresponding":false},{"id":238020,"name":"Ferdinand Althammer","orcid":"0000-0003-4508-6832","position":0,"is_corresponding":true}],"reference_count":4,"raw_metadata":null,"created_at":"2026-07-19T00:25:58.993402Z","pmid":"35199030","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}