{"doi":"10.1016/j.xpro.2021.100854","title":"An optimized ATAC-seq protocol for genome-wide mapping of active regulatory elements in primary mouse cortical neurons","abstract":"ATAC-seq is a versatile, adaptable, and widely adopted technique for mapping open chromatin regions. However, some biological systems, such as primary neurons, present unique challenges to its application. Conventional ATAC-seq would require the dissociation of the primary neurons after plating but dissociating them leads to rapid cell death and major changes in cell state, affecting ATAC-seq results. We have developed this modified ATAC-seq protocol to address this challenge for primary neurons, providing a high-quality and high-resolution accessible chromatin profile. For complete details on the use and execution of this protocol, please refer to Maor-Nof et al. (2021).","journal":"STAR Protocols","year":2021,"id":201677,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9497,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":342950,"name":"Zohar Shipony","orcid":"0000-0003-2110-8822","position":1,"is_corresponding":false},{"id":11727,"name":"Georgi K. Marinov","orcid":"0000-0003-1822-7273","position":2,"is_corresponding":false},{"id":15493,"name":"William J. Greenleaf","orcid":"0000-0003-1409-3095","position":3,"is_corresponding":false},{"id":230690,"name":"Aaron D. Gitler","orcid":"0000-0001-8603-1526","position":4,"is_corresponding":false},{"id":625929,"name":"Maya Maor-Nof","orcid":"0000-0002-8530-4497","position":0,"is_corresponding":true}],"reference_count":5,"raw_metadata":null,"created_at":"2026-07-18T23:50:56.394488Z","pmid":"34647036","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}