{"doi":"10.1016/j.xpro.2021.100486","title":"Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms","abstract":"MNase-seq (micrococcal nuclease sequencing) is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have developed a streamlined protocol for S. cerevisiae and other fungi which takes only three hours. Modified protocols were developed for wild fungi and mammalian cells. This method for rapidly producing sequencing-ready nucleosome footprints from several organisms makes MNase-seq faster and easier, with less chemical waste.","journal":"STAR Protocols","year":2021,"id":173444,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9469,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":574800,"name":"Johnathan G. Crandall","orcid":"0000-0002-9144-3135","position":1,"is_corresponding":false},{"id":575333,"name":"Thomas B. Bailey","orcid":null,"position":2,"is_corresponding":false},{"id":699312,"name":"Orion GB Banks","orcid":"0000-0001-6482-0030","position":3,"is_corresponding":false},{"id":711207,"name":"Kona N. Orlandi","orcid":"0000-0002-6426-4764","position":4,"is_corresponding":false},{"id":574801,"name":"Vi N. Truong","orcid":"0000-0003-3303-0380","position":5,"is_corresponding":false},{"id":574799,"name":"Drake A. Donovan","orcid":"0000-0002-1595-9025","position":6,"is_corresponding":false},{"id":711208,"name":"Grace L. Waddell","orcid":"0000-0002-9614-9525","position":7,"is_corresponding":false},{"id":337809,"name":"Elizabeth T. Wiles","orcid":"0000-0001-7269-7466","position":8,"is_corresponding":false},{"id":653790,"name":"Scott D. Hansen","orcid":"0000-0001-7005-6200","position":9,"is_corresponding":false},{"id":337810,"name":"Eric U. Selker","orcid":"0000-0001-6465-0094","position":10,"is_corresponding":false},{"id":574804,"name":"Jeffrey N. McKnight","orcid":"0000-0002-1066-1879","position":11,"is_corresponding":false},{"id":574803,"name":"Laura E. McKnight","orcid":"0000-0002-4322-3066","position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-18T23:46:49.903770Z","pmid":"34041500","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}