{"doi":"10.1016/j.xpro.2020.100071","title":"Convenient Protocol for Production and Purification of Clostridioides difficile Spores for Germination Studies","abstract":", an obligate anaerobic bacterium, causes infections leading to prolonged diarrhea. The bacterium produces dormant spores that can withstand an aerobic environment, resulting in easy environmental transfer. Here, we present a convenient sporulation and purification protocol that can be practiced in any lab setting using a portable anaerobic glove bag. This protocol also optimizes existing cell growth methods and presents a detailed trouble shooting guide. This protocol is a modification of those previously reported by Edwards and McBride (2016) and Shen et al. (2016).","journal":"STAR Protocols","year":2020,"id":74024,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9575,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":387856,"name":"Clayton Evert","orcid":"0000-0002-0237-3738","position":1,"is_corresponding":false},{"id":387857,"name":"Peter I. Dosa","orcid":"0000-0002-0081-3867","position":2,"is_corresponding":false},{"id":320937,"name":"Alexander Khoruts","orcid":"0000-0002-3205-3188","position":3,"is_corresponding":false},{"id":387858,"name":"Michael J. Sadowsky","orcid":"0000-0001-8779-2781","position":4,"is_corresponding":false},{"id":389316,"name":"Melissa Weldy","orcid":null,"position":0,"is_corresponding":true}],"reference_count":4,"raw_metadata":null,"created_at":"2026-07-18T21:45:52.171053Z","pmid":"33111107","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}