{"doi":"10.1016/j.xjon.2022.05.004","title":"Reply from authors: Toward physiologically meaningful biomechanical parameters from ex vivo biaxial testing","abstract":"The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. Stemming from the Commentary by Tang and colleagues1Tang M. Simmons C.A. Chung J.C.Y. Searching for a physiologically meaningful parameter for aortic biomechanics—is energy loss the way?.J Thorac Cardiovasc Surg Open. 2022; 10: 123-124Scopus (2) Google Scholar on our article,2Nightingale M. Gregory A. Sigaeva T. Dobson G.M. Fedak P.W.M. Appoo J.J. et al.Biomechanics in ascending aortic aneurysms correlate with tissue composition and strength.J Thorac Cardiovasc Surg Open. 2022; 9: 1-10Scopus (6) Google Scholar we were excited to be invited to elaborate more on the biaxial ex vivo biomechanical parameters for assessing ascending aortic aneurysms. Particularly, Tang and colleagues2Nightingale M. Gregory A. Sigaeva T. Dobson G.M. Fedak P.W.M. Appoo J.J. et al.Biomechanics in ascending aortic aneurysms correlate with tissue composition and strength.J Thorac Cardiovasc Surg Open. 2022; 9: 1-10Scopus (6) Google Scholar raise an excellent point regarding the limitations of ex vivo testing. Some researchers have tried to estimate an in vivo baseline strain to calculate the modulus of elasticity, this is the case for some of previous work by Chung and colleagues,3Chung J.C. Wong E. Tang M. Eliathamby D. Forbes T.L. Butany J. et al.Biomechanics of aortic dissection: a comparison of aortas associated with bicuspid and tricuspid aortic valves.J Am Heart Assoc. 2020; 9e016715Crossref Scopus (23) Google Scholar who used a physiological strain estimate of 10%. To our knowledge, there is yet no definitive method for calculating a physiological baseline stress/strain. The Law of Laplace, used to calculate stress, likely overestimates the pressure by not considering the transmural pressure within the aorta and there is currently no accurate way to assess residual strains. As such, we have chosen to move away from defining a modulus/stiffness at a specific value (not to mention that these values would need to be patient- and even region-specific) and toward defined features of the aortic material itself such as the low strain tangential modulus (LTM) and stress at the transition zone (TZo). LTM and TZo were chosen as physiologically meaningful given that healthy aortic tissue is not likely to reach the high strain modulus at the displacement control protocol used in this article, which is set to cause 60% biaxial strain in grip-to-grip distances. For our aneurysm samples, only 25 out of 41 samples presented full TZos. Therefore, comparing LTM and TZo seems more meaningful than comparing tangent moduli at a fixed strain because they are likely to not be equivalent measures for aneurysms with different stages of pathology progression. Additionally, these properties are believed to correlate with the microstructure of the tissue and may provide more information beyond a general assessment of stiffening. Tracking changes in LTM over time may shed light on the level of degradation of the elastic lamellae, whereas a lower TZo may illustrate structural changes to collagen.2Nightingale M. Gregory A. Sigaeva T. Dobson G.M. Fedak P.W.M. Appoo J.J. et al.Biomechanics in ascending aortic aneurysms correlate with tissue composition and strength.J Thorac Cardiovasc Surg Open. 2022; 9: 1-10Scopus (6) Google Scholar The energy loss in our article2Nightingale M. Gregory A. Sigaeva T. Dobson G.M. Fedak P.W.M. Appoo J.J. et al.Biomechanics in ascending aortic aneurysms correlate with tissue composition and strength.J Thorac Cardiovasc Surg Open. 2022; 9: 1-10Scopus (6) Google Scholar was calculated from the machine grip-to-grip measurements versus local central dot deformation used to calculate LTM and TZo. As such, our measurement of energy loss was determined with th","journal":"JTCVS Open","year":2022,"id":301228,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9677,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":699087,"name":"Taisiya Sigaeva","orcid":"0000-0001-6764-1089","position":1,"is_corresponding":false},{"id":699089,"name":"Elena S. Di Martino","orcid":"0000-0002-8005-9445","position":2,"is_corresponding":false},{"id":699895,"name":"Miriam Nightingale","orcid":null,"position":0,"is_corresponding":true}],"reference_count":6,"raw_metadata":null,"created_at":"2026-07-19T00:32:02.193874Z","pmid":"36004239","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}