{"doi":"10.1016/j.xgen.2024.100720","title":"Adult single-nucleus neuronal transcriptomes of insulin signaling mutants reveal regulators of behavior and learning","abstract":"Gene expression in individual neurons can change during development to adulthood and can have large effects on behavior. Additionally, the insulin/insulin-like signaling (IIS) pathway regulates many of the adult functions of Caenorhabditis elegans, including learning and memory, via transcriptional changes. We used the deep resolution of single-nucleus RNA sequencing to define the adult transcriptome of each neuron in wild-type and daf-2 mutants, revealing expression differences between L4 larval and adult neurons in chemoreceptors, synaptic genes, and learning/memory genes. We used these data to identify adult new AWC-specific regulators of chemosensory function that emerge upon adulthood. daf-2 gene expression changes correlate with improved cognitive functions, particularly in the AWC sensory neuron that controls learning and associative memory; behavioral assays of AWC-specific daf-2 genes revealed their roles in cognitive function. Combining technology and functional validation, we identified conserved genes that function in specific adult neurons to control behavior, including learning and memory.","journal":"Cell Genomics","year":2024,"id":433415,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.926,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1071760,"name":"Yifei Weng","orcid":"0009-0003-6229-8631","position":1,"is_corresponding":false},{"id":256013,"name":"Rachel Kaletsky","orcid":"0000-0002-7176-9154","position":2,"is_corresponding":false},{"id":1071758,"name":"Morgan E. Stevenson","orcid":"0000-0002-5987-8358","position":3,"is_corresponding":false},{"id":784963,"name":"Rebecca S. Moore","orcid":"0000-0003-3155-3436","position":4,"is_corresponding":false},{"id":327988,"name":"Shiyi Zhou","orcid":"0000-0002-3824-2588","position":5,"is_corresponding":false},{"id":256015,"name":"Coleen T. Murphy","orcid":"0000-0002-8257-984X","position":6,"is_corresponding":false},{"id":424636,"name":"Jonathan St. Ange","orcid":"0009-0001-2319-8174","position":0,"is_corresponding":true}],"reference_count":94,"raw_metadata":null,"created_at":"2026-07-19T01:59:44.444627Z","pmid":"39637862","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}