{"doi":"10.1016/j.visres.2024.108456","title":"Biomechanic, proteomic and miRNA transcriptional changes in the trabecular meshwork of primates injected with intravitreal triamcinolone","abstract":"Although biomechanical changes of the trabecular meshwork (TM) are important to the pathogenesis of glucocorticoids-induced ocular hypertension (GC-OHT), there is a knowledge gap in the underlying molecular mechanisms of the development of it. In this study, we performed intravitreal triamcinolone injection (IVTA) in one eye of 3 rhesus macaques. Following IVTA, we assessed TM stiffness using atomic force microscopy and investigated changes in proteomic and miRNA expression profiles. One of 3 macaques developed GC-OHT with a difference in intraocular pressure of 4.2 mmHg and a stiffer TM with a mean increase in elastic moduli of 0.60 kPa versus the non-injected control eye. In the IVTA-treated eyes, proteins associated with extracellular matrix remodeling, cytoskeletal rearrangement, and mitochondrial oxidoreductation were significantly upregulated. The significantly upregulated miR-29b and downregulated miR-335-5p post-IVTA supported the role of oxidative stress and mitophagy in the GC-mediated biomechanical changes in TM, respectively. The significant upregulation of miR-15/16 cluster post-IVTA may indicate a resultant TM cell apoptosis contributing to the increase in outflow resistance. Despite the small sample size, these results expand our knowledge of GC-mediated responses in the TM and furthermore, may help explain steroid responsiveness in clinical settings.","journal":"Vision Research","year":2024,"id":459780,"datarank":0.26364950699292206,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.02223382012780698,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.02223382012780698,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":4,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":254823,"name":"Vijay Krishna Raghunathan","orcid":"0000-0003-1702-591X","position":1,"is_corresponding":false},{"id":1012991,"name":"Raneesh Ramarapu","orcid":null,"position":2,"is_corresponding":false},{"id":445260,"name":"Ala Moshiri","orcid":"0000-0002-4277-8094","position":3,"is_corresponding":false},{"id":260772,"name":"Glenn Yiu","orcid":"0000-0003-3061-3310","position":4,"is_corresponding":false},{"id":407466,"name":"M. Isabel Casanova","orcid":"0000-0003-2337-5051","position":5,"is_corresponding":false},{"id":889979,"name":"Krista Cosert","orcid":null,"position":6,"is_corresponding":false},{"id":1229104,"name":"Michelle E Mccorkell","orcid":null,"position":7,"is_corresponding":false},{"id":692180,"name":"Brian C. Leonard","orcid":"0000-0003-2080-8480","position":8,"is_corresponding":false},{"id":246470,"name":"Sara M. Thomasy","orcid":"0000-0001-5617-9677","position":9,"is_corresponding":false},{"id":445258,"name":"Sangwan Park","orcid":"0000-0003-0381-0386","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:03:59.428859Z","pmid":"38991466","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}