{"doi":"10.1016/j.virol.2017.04.001","title":"Inhibition of CRM1-mediated nuclear export of influenza A nucleoprotein and nuclear export protein as a novel target for antiviral drug development","abstract":null,"journal":"Virology","year":2017,"id":620238,"datarank":0.4566783656585135,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.0,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1601076,"name":"Takafumi Mano","orcid":null,"position":1,"is_corresponding":false},{"id":174025,"name":"Michinori Kakisaka","orcid":null,"position":2,"is_corresponding":false},{"id":528384,"name":"Hirotaka Sato","orcid":"0000-0002-8321-9731","position":3,"is_corresponding":false},{"id":967695,"name":"Yasumitsu Kondoh","orcid":"0000-0002-3481-5481","position":4,"is_corresponding":false},{"id":812350,"name":"Hiroyuki Osada","orcid":"0000-0002-3606-4925","position":5,"is_corresponding":false},{"id":759699,"name":"Osamu Kotani","orcid":"0000-0003-1665-6824","position":6,"is_corresponding":false},{"id":515191,"name":"Masaru Yokoyama","orcid":"0000-0003-1641-0365","position":7,"is_corresponding":false},{"id":1243378,"name":"Hironori Sato","orcid":"0000-0003-3217-3742","position":8,"is_corresponding":false},{"id":174026,"name":"Yoko Aida","orcid":null,"position":9,"is_corresponding":false},{"id":174024,"name":"Nopporn Chutiwitoonchai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Inhibition of CRM1-mediated nuclear export of influenza A nucleoprotein and nuclear export protein as a novel target for antiviral drug development","abstract":"An anti-influenza compound, DP2392-E10 based on inhibition of the nuclear export function of the viral nucleoprotein-nuclear export signal 3 (NP-NES3) domain was successfully identified by our previous high-throughput screening system. Here, we demonstrated that DP2392-E10 exerts its antiviral effect by inhibiting replication of a broad range of influenza A subtypes. In regard to the molecular mechanism, we revealed that DP2392-E10 inhibits nuclear export of both viral NP and nuclear export protein (NEP). More specifically, in vitro pull-down assays revealed that DP2392-E10 directly binds cellular CRM1, which mediates nuclear export of NP and NEP. In silico docking suggested that DP2392-E10 binds at a region close to the HEAT9 and HEAT10 domains of CRM1. Together, these results indicate that the CRM1-mediated nuclear export function of influenza virus represents a new potential target for antiviral drug development, and also provide a core structure for a novel class of inhibitors that target this function.","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28399435","pmcid":"PMC7111614","openalex_id":"https://openalex.org/W2604112880","authors":[],"funders":[{"funder_name":"Ministry of Education, Culture, Sports, Science and Technology, Japan","grant_id":"DP2392-E10","title":null},{"funder_name":"Ministry of Agriculture, Forestry and Fisheries of Japan","grant_id":"1340642","title":null},{"funder_name":"NCCDPHP CDC HHS","grant_id":"U58 DP002392","title":null},{"funder_name":"Japan Society for the Promotion of Science (JSPS","grant_id":"","title":null}],"total_grants":4,"fwci":1.1153,"citation_percentile":0.76211952,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":3},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":2}],"oa_status":"bronze","license":"http://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S0042682217301022/pdf","host_type":"journal"},{"url":"https://www.sciencedirect.com/science/article/pii/S0042682217301022/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0042682217301022?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0042682217301022?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.virol.2017.04.001","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28399435","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7111614","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7111614","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7111614?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["RNA Research and Splicing","interferon and immune responses","Nuclear Structure and Function","Active Transport, Cell Nucleus","Antiviral Agents","Cell Line","Cell Nucleus","Humans","Influenza A virus","Influenza, Human","Karyopherins","Nucleoproteins","Receptors, Cytoplasmic and Nuclear","Exportin 1 Protein"],"mesh_terms":["Exportin 1 Protein","Antiviral Agents","Cell Line","Cell Nucleus","Humans","Influenza, Human","Nucleoproteins","Influenza A virus","Receptors, Cytoplasmic and Nuclear","Active Transport, Cell Nucleus","Karyopherins"],"keywords":["Nuclear export signal","Nucleoprotein","Biology","Nuclear transport","In silico","Nuclear protein","Viral protein","Viral replication","Influenza A virus","Cell biology","Virology","Virus","Cell nucleus","Genetics","Transcription factor","Gene","Nucleus","Nuclear Export Protein","Chromosome Region Maintenance 1"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T10:28:53.219018Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}