{"doi":"10.1016/j.tpr.2021.100079","title":"Single center experience comparing two clinically available donor derived cell free DNA tests and review of literature","abstract":"The introduction of assays for donor-derived cell-free DNA into clinical transplant medicine has provided an additional method to assess allograft health. We compared the performance of two of these assays, Prospera™ and AlloSure®. A series of 15 paired-samples from individual kidney transplant recipients were tested using both Prospera™ and AlloSure® assays simultaneously. Test performances were determined using the company cutoff of >1% to indicate active rejection. Additional analysis was also performed using a cutoff of 0.5%. Acute rejections were all confirmed by biopsy. There was one discordant result for 15 paired-samples when using a cutoff level of 1%, and results were concordant using a cutoff level of 0.5%. Seven biopsy were performed, six of which showed rejection. Using cutoff of 1%, Prospera™ identified 80% (4/5) of T cell-mediated rejections (TCMR) compared to 60% for AlloSure® (3/5). Both assays recognized the only case of antibody-mediated rejection. When using cutoff level of 0.5%, both assays correctly identified all cases of rejection. In this cohort, the two tests showed different sensitivities when using the validated cutoff of 1% dd-cfDNA, and the same sensitivity when using a lower cutoff of 0.5%. The result accuracy was confirmed by kidney biopsy.","journal":"Transplantation Reports","year":2021,"id":195627,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":767594,"name":"Karid Nieves-Borrero","orcid":null,"position":1,"is_corresponding":false},{"id":767004,"name":"Piyavadee Homkrailas","orcid":"0000-0002-6846-2100","position":2,"is_corresponding":false},{"id":767005,"name":"Sabrina Lee","orcid":"0000-0002-4660-514X","position":3,"is_corresponding":false},{"id":767006,"name":"Gabriel M. Danovitch","orcid":"0000-0002-6114-8709","position":4,"is_corresponding":false},{"id":705072,"name":"Suphamai Bunnapradist","orcid":"0000-0002-1668-2005","position":5,"is_corresponding":false},{"id":520836,"name":"Erik L. Lum","orcid":"0000-0001-9495-8225","position":0,"is_corresponding":true}],"reference_count":12,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:50:07.691929Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}