{"doi":"10.1016/j.tiv.2020.104832","title":"An international validation study of the IL-2 Luc assay for evaluating the potential immunotoxic effects of chemicals on T cells and a proposal for reference data for immunotoxic chemicals","abstract":"To evaluate the immunotoxic effects of xenobiotics, we have established the Multi-ImmunoTox assay, in which three stable reporter cell lines are used to evaluate the effects of chemicals on the IL-2, IFN-γ, IL-1β and IL-8 promoters. Here, we report the official validation study of the IL-2 luciferase assay (IL-2 Luc assay). In the Phase I study that evaluated five coded chemicals in three sets of experiments, the average within-laboratory reproducibility was 86.7%. In the Phase II study, 20 coded chemicals were evaluated at multiple laboratories. In the combined results of the Phase I and II studies, the between-laboratory reproducibility was 80.0%. These results suggested that the IL-2 Luc assay was reproducible both between and within laboratories. To determine the predictivity, we collected immunotoxicological information and constructed the reference data by classifying the chemical into immunotoxic compounds targeting T cells or others according to previously reported criteria. When compared with the reference data, the average predictivity of the Phase I and II studies was 75.0%, while that of additional 60 chemicals examined by the lead laboratory was 82.5%. Although the IL-2 Luc assay alone is not sufficient to predict immunotoxicity, it will be a useful tool when combined with other immune tests.","journal":"Toxicology in Vitro","year":2020,"id":77234,"datarank":0.4493598410330987,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.0,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8414,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":402382,"name":"Rie Yasuno","orcid":"0000-0003-0108-9281","position":1,"is_corresponding":false},{"id":402383,"name":"Mika Watanabe","orcid":"0000-0002-0075-0851","position":2,"is_corresponding":false},{"id":372699,"name":"Miwako Kobayashi","orcid":null,"position":3,"is_corresponding":false},{"id":403490,"name":"Tomoko Iwaki","orcid":null,"position":4,"is_corresponding":false},{"id":403491,"name":"Chizu Fujimura","orcid":null,"position":5,"is_corresponding":false},{"id":402384,"name":"Yoshihiro Ohmiya","orcid":"0000-0002-4944-509X","position":6,"is_corresponding":false},{"id":403492,"name":"Kohji Yamakage","orcid":null,"position":7,"is_corresponding":false},{"id":402385,"name":"Yoshihiro Nakajima","orcid":"0000-0002-7422-730X","position":8,"is_corresponding":false},{"id":403493,"name":"Mayumi Kobayashi","orcid":null,"position":9,"is_corresponding":false},{"id":403494,"name":"Nana Mashimo","orcid":null,"position":10,"is_corresponding":false},{"id":403495,"name":"Yumi Takagi","orcid":null,"position":11,"is_corresponding":false},{"id":402386,"name":"Takashi Omori","orcid":"0000-0002-4460-9032","position":12,"is_corresponding":false},{"id":402387,"name":"Emanuela Corsini","orcid":"0000-0002-6927-5956","position":13,"is_corresponding":false},{"id":374780,"name":"Dori R. Germolec","orcid":"0000-0003-4072-1956","position":14,"is_corresponding":false},{"id":402388,"name":"Tomoaki Inoue","orcid":"0000-0002-9693-5040","position":15,"is_corresponding":false},{"id":403496,"name":"Erwin L. Rogen","orcid":null,"position":16,"is_corresponding":false},{"id":402389,"name":"Hajime Kojima","orcid":"0000-0002-4485-1304","position":17,"is_corresponding":false},{"id":402390,"name":"Setsuya Aiba","orcid":"0000-0003-0632-2388","position":18,"is_corresponding":false},{"id":402381,"name":"Yutaka Kimura","orcid":"0000-0002-1823-9234","position":0,"is_corresponding":true}],"reference_count":15,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:48:50.085103Z","pmid":"32200032","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}