{"doi":"10.1016/j.tibtech.2024.10.011","title":"Intracellularly synthesized ssDNA for continuous genome engineering","abstract":null,"journal":"Trends in Biotechnology","year":2025,"id":640230,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1663864,"name":"Yingjia Pan","orcid":null,"position":1,"is_corresponding":false},{"id":831198,"name":"Yu Zhang","orcid":"0000-0001-5969-2388","position":2,"is_corresponding":false},{"id":1663865,"name":"Chang Dong","orcid":null,"position":3,"is_corresponding":false},{"id":249675,"name":"Lei Huang","orcid":"0000-0002-1996-944X","position":4,"is_corresponding":false},{"id":1650880,"name":"Jiazhang Lian","orcid":"0000-0001-9784-9876","position":5,"is_corresponding":false},{"id":1626587,"name":"Wenqian Liu","orcid":"0000-0002-4930-8116","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Intracellularly synthesized ssDNA for continuous genome engineering","abstract":"Despite the prevalence of genome editing tools, there are still some limitations in dynamic and continuous genome editing. In vivo single-stranded DNA (ssDNA)-mediated genome mutation has emerged as a valuable and promising approach for continuous genome editing. In this review, we summarize the various types of intracellular ssDNA production systems and notable achievements in genome engineering in both prokaryotic and eukaryotic cells. We also review progress in the development of applications based on retron-based systems, which have demonstrated significant potential in molecular recording, multiplex genome editing, high-throughput functional variant screening, and gene-specific continuous in vivo evolution. Furthermore, we discuss the major challenges of ssDNA-mediated continuous genome editing and its prospects for future applications.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39537537","pmcid":null,"openalex_id":"https://openalex.org/W4404268286","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"32300053","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32200052","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"22278361","title":null},{"funder_name":"Fundamental Research Funds for the Central Universities","grant_id":"226-2022-00214","title":null},{"funder_name":"Fundamental Research Funds for the Central Universities","grant_id":"226-2023-00085","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2021YFC2103200","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2023YFC3402400","title":null},{"funder_name":"Fundamental Research Funds for the Provincial Universities of Zhejiang","grant_id":"","title":null}],"total_grants":8,"fwci":1.7772,"citation_percentile":0.85329697,"influential_citations":0,"citation_trend":[{"year":2025,"count":7},{"year":2026,"count":4}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0167779924002932?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0167779924002932?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.tibtech.2024.10.011","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39537537","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","Animal Genetics and Reproduction","RNA and protein synthesis mechanisms","DNA, Single-Stranded","Gene Editing","CRISPR-Cas Systems"],"mesh_terms":["Gene Editing","DNA, Single-Stranded","CRISPR-Cas Systems"],"keywords":["Genome","Computational biology","DNA","Genome engineering","Nanotechnology","Biology","Chemistry","Genetics","Gene","Genome editing","Materials science","Retron","Continuous In Vivo Evolution","Intracellular Ssdna Production"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T08:40:45.470313Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}