{"doi":"10.1016/j.thromres.2007.05.021","title":"Pro-urokinase up-regulates the expression of urokinase-type plasminogen activator (u-PA) in human pulmonary arterial endothelial cells","abstract":null,"journal":"Thrombosis Research","year":2008,"id":639078,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":804100,"name":"Chen Wang","orcid":"0009-0005-6441-5939","position":1,"is_corresponding":false},{"id":1660241,"name":"Yuanhua Yang","orcid":null,"position":2,"is_corresponding":false},{"id":1096581,"name":"Xiaoli Hou","orcid":"0000-0001-5299-6725","position":3,"is_corresponding":false},{"id":242464,"name":"Jun Wang","orcid":"0000-0002-8104-7470","position":4,"is_corresponding":false},{"id":831967,"name":"Yi Liu","orcid":"0000-0002-7782-4548","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Pro-urokinase up-regulates the expression of urokinase-type plasminogen activator (u-PA) in human pulmonary arterial endothelial cells","abstract":"The fibrinolytic function of endothelial cells plays an important role in the pathophysiology of pulmonary vascular diseases. In this study, the effects of pro-urokinase, a new thrombolytic drug that is currently being tested for the treatment of pulmonary embolism, on the expression of urokinase-type plasminogen activator (u-PA) and u-PA receptor (u-PAR) were assessed. The role of u-PAR was also investigated. Immunocytofluorescence and RT-PCR techniques were employed. In normal human pulmonary arterial endothelial cells (HPAECs), the expression levels of u-PA and u-PAR were very low. Incubation with pro-urokinase up-regulated u-PA expression at both the mRNA level and the protein level; however, the expression of u-PAR was not affected. The effect of pro-urokinase induction was totally inhibited by the release of u-PAR from the HPAECs' surface with PLC. This result suggests that the combination of u-PA with u-PAR may be a critical pathway for the induction of u-PA expression.","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17640719","pmcid":null,"openalex_id":"https://openalex.org/W2114135330","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.12853193,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2022,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0049384807002356?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0049384807002356?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.thromres.2007.05.021","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17640719","host_type":"repository"}],"fields_of_study":["Protease and Inhibitor Mechanisms","Blood Coagulation and Thrombosis Mechanisms","Blood properties and coagulation"],"mesh_terms":["Cells, Cultured","Humans","Pulmonary Artery","Recombinant Proteins","RNA, Messenger","Urokinase-Type Plasminogen Activator","Up-Regulation","Gene Expression Regulation, Enzymologic","Endothelial Cells"],"keywords":["Urokinase","Plasminogen activator","Urokinase receptor","Activator (genetics)","Pulmonary embolism","Incubation","Medicine","Messenger RNA","Receptor","Pharmacology","Immunology","Endocrinology","Internal medicine","Chemistry","Molecular biology","Biology","Biochemistry","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T22:25:59.542748Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}