{"doi":"10.1016/j.tet.2014.12.056","title":"Site directed nitroxide spin labeling of oligonucleotides for NMR and EPR studies","abstract":null,"journal":"Tetrahedron","year":2015,"id":667051,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1158910,"name":"Roland Gamsjaeger","orcid":"0000-0003-1095-2569","position":1,"is_corresponding":false},{"id":129406,"name":"Marylène Vandevenne","orcid":null,"position":2,"is_corresponding":false},{"id":1159380,"name":"Liza Cubeddu","orcid":null,"position":3,"is_corresponding":false},{"id":78312,"name":"Joel P. Mackay","orcid":"0000-0001-7508-8033","position":4,"is_corresponding":false},{"id":308207,"name":"Nicholas E. Shepherd","orcid":"0000-0001-6234-4456","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Site directed nitroxide spin labeling of oligonucleotides for NMR and EPR studies","abstract":"Nitroxide labels are useful probes of biomolecule structure that can be detected by NMR and EPR spectroscopy. Although many methods exist for labeling oligonucleotides with nitroxides, most require reagents that are expensive or laborious to prepare. A simpler approach is described herein using commercially available phosphorothioate oligonucleotides and 2-(3-iodoacetamidomethyl)-PROXYL. We describe semi-optimized conditions for labeling DNA and RNA oligonucleotides and methodology for purifying and identifying these reagents by MALDI-MS. The nitroxide label showed some propensity to hydrolyze at high temperature and over prolonged periods at room temperature. Nitroxide-labeled DNA oligonucleotides gave characteristic EPR spectra and caused the disappearance of bound protein signals in 15N HSQC spectra consistent with the paramagnetic relaxation enhancement (PRE) effect.","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W2026618861","authors":[],"funders":[{"funder_name":"Australian Research Council","grant_id":"DE120102857","title":"Innovative chemical tools for the isolation, biochemical and structural analysis of biological macromolecular assemblies"}],"total_grants":1,"fwci":1.0734,"citation_percentile":0.83837265,"influential_citations":0,"citation_trend":[{"year":2017,"count":3},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2024,"count":1},{"year":2025,"count":3}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.sciencedirect.com/science/article/pii/S0040402014017608/pdf","host_type":"journal"},{"url":"https://www.sciencedirect.com/science/article/pii/S0040402014017608/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0040402014017608?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0040402014017608?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.tet.2014.12.056","host_type":"journal"},{"url":"https://orbi.uliege.be/handle/2268/190171","host_type":"repository"},{"url":"http://dx.doi.org/10.1016/j.tet.2014.12.056","host_type":""},{"url":"https://espace.library.uq.edu.au/view/UQ:387067","host_type":""},{"url":"https://api.library.uq.edu.au/view/UQ:387067","host_type":""},{"url":"https://dx.doi.org/10.1016/j.tet.2014.12.056","host_type":""},{"url":"https://hdl.handle.net/2268/190171","host_type":""},{"url":"https://doi.org/https://doi.org/10.1016/j.tet.2014.12.056","host_type":""}],"fields_of_study":["Electron Spin Resonance Studies","Advanced NMR Techniques and Applications","Lanthanide and Transition Metal Complexes","0301 basic medicine","01 natural sciences","0104 chemical sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Nitroxide mediated radical polymerization","Chemistry","Oligonucleotide","Site-directed spin labeling","Electron paramagnetic resonance","Biomolecule","Reagent","Multiplex","Heteronuclear single quantum coherence spectroscopy","DNA","Nuclear magnetic resonance spectroscopy","Combinatorial chemistry","Nuclear magnetic resonance","Stereochemistry","Biochemistry","Organic chemistry","Bioinformatics","Polymerization","1303 Biochemistry","3002 Drug Discovery","Nitroxide","500","Paramagnetic relaxation enhancement","030499 - Medicinal and Biomolecular Chemistry not elsewhere classified","540","Biochimie, biophysique & biologie moléculaire","Life sciences","Drug Discovery","Sciences du vivant","Phosphorothioate","Electron paramagnetic resonance spectroscopy","Biochemistry, biophysics & molecular biology","1605 Organic Chemistry"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T16:27:03.778615Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}