{"doi":"10.1016/j.synbio.2025.09.014","title":"Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization","abstract":null,"journal":"Synthetic and Systems Biotechnology","year":2026,"id":673836,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1760574,"name":"Guang-Qing Du","orcid":null,"position":1,"is_corresponding":false},{"id":1760575,"name":"Xu-Yang Huang","orcid":null,"position":2,"is_corresponding":false},{"id":1760576,"name":"Zheng-Zi Ji","orcid":null,"position":3,"is_corresponding":false},{"id":1760577,"name":"Si-Riguleng Qian","orcid":null,"position":4,"is_corresponding":false},{"id":1760578,"name":"Lin-Xia Liu","orcid":"0000-0001-9957-0545","position":5,"is_corresponding":false},{"id":819257,"name":"Dawei Zhang","orcid":"0000-0002-5857-394X","position":6,"is_corresponding":false},{"id":1760572,"name":"Ai-Tong Jiang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization","abstract":"Vitamin B 6 is a water-soluble B vitamin family, which is essential for various physiological functions, including amino acid metabolism, neurotransmitter synthesis, and hemoglobin synthesis. The biosynthesis of pyridoxine (PN), a commercial form of vitamin B 6 , through microbial fermentation has garnered widespread attention owing to its environmentally friendly and safe production methods, as well as its mild reaction conditions. However, the low yield of natural strains limits their application. This study focused on constructing a high-yielding strain of PN through pathway engineering and process optimization. Firstly, five key deoxyxylulose-5-phosphate-dependent pathway genes ( epd, pdxB, serC, pdxA, and pdxJ ) were overexpressed in Bacillus subtilis ARTP, which improved the PN titer by 3.2-fold to 2.9 mg/L. Subsequently, pdxST genes involved in the DXP-independent pathway were screened from various strains. Ribosome binding site (RBS) sequences were optimized to optimize their expression, which further increased the PN titer to 24.6 mg/L. Finally, systematic medium optimization was identified as a critical strategy for enhancing PN biosynthesis, leading to a remarkable 1.8-fold improvement in PN production. Under optimized fermentation conditions, the engineered strain achieved a PN titer of 174.6 mg/L in fed-batch fermentation, which represents the highest level reported to date in B . subtilis . Overall, this study presents an effective strategy combining pathway engineering and medium optimization for significantly improving PN production, offering valuable insights for the industrial development of PN biosynthesis.","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41089825","pmcid":"PMC12517072","openalex_id":"https://openalex.org/W4414303186","authors":[],"funders":[{"funder_name":"National Science Fund for Distinguished Young Scholars","grant_id":"22325807","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2022YFC2106100","title":null},{"funder_name":"Chinese Academy of Sciences","grant_id":"306GJHZ2023019GC","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32200049","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"22208368","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"22178372","title":null}],"total_grants":6,"fwci":2.0887,"citation_percentile":0.87806426,"influential_citations":0,"citation_trend":[{"year":2026,"count":8}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://doi.org/10.1016/j.synbio.2025.09.014","host_type":"journal"},{"url":"https://doi.org/10.1016/j.synbio.2025.09.014","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2405805X25001553?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2405805X25001553?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41089825","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12517072","host_type":"repository"},{"url":"https://doaj.org/article/128e5cb3324840d5bb772bf99f756a94","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC12517072","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12517072?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Enzyme Structure and Function","Enzyme Production and Characterization","Biochemical and Molecular Research"],"mesh_terms":[],"keywords":["Bacillus subtilis","Metabolic engineering","Pyridoxine","Fermentation","Mutant","Metabolic pathway","Biosynthesis","Amino acid","medium optimization","Dxp-Independent Pathway"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T14:39:08.939724Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}